Qiang Na, Tang Shuo, Shi Xiao-jun, Li Hao, Ma Yi-hong, Tao Hai-xia, Lin Qiang
a Department of Chemical Engineering , Huizhou University , Huizhou , China.
b Department of Orthopaedics , West China Hospital, Sichuan University , Chengdu , China.
J Biomater Sci Polym Ed. 2016;27(3):191-201. doi: 10.1080/09205063.2015.1114308. Epub 2015 Dec 20.
An ideal scaffold should mimic the advantageous characteristics of a natural extracellular matrix for cell attachment, proliferation, and differentiation. In this study, well-defined block copolymer with functional groups was synthesized. The structure of the block copolymer was characterized by nuclear magnetic resonance, gel permeation chromatography, and differential scanning calorimetry. Thermally induced phase separation was employed to fabricate nano-fibrous scaffolds based on the synthesized block copolymer. The scaffold, with fiber diameter ranging from 400 to 500 nm, was fabricated for in vitro culture of PC12 cells. The carboxyl groups on the side chain resulted in increased hydrophilicity of nano-fibrous scaffolds and enhanced cell proliferation. In addition, this scaffold structure was beneficial in directing the growth of regenerating axons in nerve tissue engineering. Results of 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay and scanning electron microscopy confirmed that the nano-fibrous scaffolds with functional groups were suitable for PC12 cells growth. Moreover, the carboxyl groups were suitable for coupling with biological signals. Thus, the nano-fibrous scaffolds have potential applications in tissue engineering.
理想的支架应模仿天然细胞外基质的有利特性，以促进细胞附着、增殖和分化。在本研究中，合成了具有官能团的明确嵌段共聚物。通过核磁共振、凝胶渗透色谱和差示扫描量热法对嵌段共聚物的结构进行了表征。采用热致相分离法，基于合成的嵌段共聚物制备了纳米纤维支架。该支架纤维直径为400至500纳米，用于PC12细胞的体外培养。侧链上的羧基增加了纳米纤维支架的亲水性并促进了细胞增殖。此外，这种支架结构有利于引导神经组织工程中再生轴突的生长。3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)测定和扫描电子显微镜结果证实，具有官能团的纳米纤维支架适合PC12细胞生长。此外，羧基适合与生物信号偶联。因此，纳米纤维支架在组织工程中具有潜在的应用价值。
