Golafshan Nasim, Gharibi Hamidreza, Kharaziha Mahshid, Fathi Mohammadhossein
Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Biofabrication. 2017 Apr 28;9(2):025008. doi: 10.1088/1758-5090/aa68ed.
The aim of this study was to develop a novel double network scaffold composed of polycaprolactone fumarate (PCLF) and eggshell membrane (ESM) (ESM:PCLF) by using the vacuum infiltration method. Compared to ESM, the mechanical properties of double network scaffold were significantly improved, depending on the solvents applied for double network scaffold formation; acetic acid and dichloromethane. Noticeably, the toughness and strength of double network scaffold prepared using acetic acid were significantly improved compared to ESM (26.6 and 25 times, respectively) attributed to the existence of hydrophilic functional groups in acetic acid which made ESM flexible to absorb further PCLF solution. To assess the effect of double network formation on the biological behavior of ESM, the attachment, proliferation and spreading of PC12 cells cultured on the ESM:PCLF scaffolds were evaluated. Results revealed that the number of cells attached on double network ESM:PCLF scaffold were nearly similar to ESM and significantly higher than that of on the tissue culture plate (2.6 times) and PCLF film (1.7 times). It is envisioned that the offered ESM:PCLF double network scaffold might have great potential to develop the constructs for nerve regeneration.
本研究的目的是通过真空浸润法开发一种由聚己内酯富马酸酯（PCLF）和蛋壳膜（ESM）组成的新型双网络支架（ESM:PCLF）。与ESM相比，双网络支架的机械性能显著提高，这取决于用于形成双网络支架的溶剂；乙酸和二氯甲烷。值得注意的是，与ESM相比，使用乙酸制备的双网络支架的韧性和强度显著提高（分别提高了26.6倍和25倍），这归因于乙酸中存在亲水性官能团，使得ESM能够灵活地吸收更多的PCLF溶液。为了评估双网络形成对ESM生物学行为的影响，对在ESM:PCLF支架上培养的PC12细胞的附着、增殖和铺展进行了评估。结果显示，附着在双网络ESM:PCLF支架上的细胞数量与ESM几乎相似，且显著高于组织培养板上的细胞数量（2.6倍）和PCLF膜上的细胞数量（1.7倍）。可以设想，所提供的ESM:PCLF双网络支架在开发用于神经再生的构建体方面可能具有巨大潜力。
