Ellis E Ann
Consultant in Biological Electron Microscopy, Thomasville, Gerogia.
Microsc Res Tech. 2016 Jan;79(1):50-7. doi: 10.1002/jemt.22597. Epub 2015 Oct 30.
Quetol 651, a low viscosity epoxy resin, is miscible with alcohols, acetone, and water. It is versatile and can be used as a single epoxide or mixed with other epoxides and anhydrides. The most important characteristic is that the addition of Quetol 651 to a formulation results in a lower viscosity embedding medium and allows for good detection of antigenic activity. Properly formulated and mixed resins containing Quetol 651 have excellent sectioning properties and good beam stability. The decrease in viscosity lends to lower specific gravity of the embedding medium and less interfering electron density between specimen elements resulting in better spatial resolution. New formulations and viscosity data are presented and compared to long used, embedding formulations and the extensive uses of Quetol 651 are reviewed.
喹托 651 是一种低粘度环氧树脂，可与醇类、丙酮和水混溶。它用途广泛，可单独用作环氧化物，也可与其他环氧化物和酸酐混合使用。最重要的特性是，在配方中添加喹托 651 会形成粘度较低的包埋介质，并有助于良好地检测抗原活性。含有喹托 651 的树脂经过适当配方和混合后，具有出色的切片性能和良好的束稳定性。粘度降低使得包埋介质的比重降低，且标本元素之间的电子密度干扰减少，从而实现更好的空间分辨率。本文给出了新的配方和粘度数据，并与长期使用的包埋配方进行了比较，同时对喹托 651 的广泛用途进行了综述。
