Mollenhauer H H
Food Animal Protection Research Laboratory, USDA, Agricultural Research Service, College Station, Texas 77845-9594.
Microsc Res Tech. 1993 Dec 15;26(6):496-512. doi: 10.1002/jemt.1070260604.
Epoxy resins are the principal embedding media for the preservation of tissues to be sectioned and examined by transmission electron microscopy. Their primary advantages are good ultrastructural preservation, little or no shrinkage, ease of sectioning, and reasonable stability in the electron beam. However, epoxy resins also have disadvantages; namely, some are toxic, they may mask antigenic sites to a greater extent than do some other embedding resins, and they do not penetrate tissues as well as less viscous embedding formulations. Some unusual characteristics may also be revealed, for example, as shrinkage of organelles, as problems in poststaining sections, and as movement of tissue elements within the block and section. Some of the properties of epoxy resins are discussed in this report.
环氧树脂是用于保存组织以便通过透射电子显微镜进行切片和检查的主要包埋介质。它们的主要优点是超微结构保存良好、收缩很少或没有收缩、易于切片以及在电子束中具有合理的稳定性。然而，环氧树脂也有缺点；即，有些有毒，它们可能比其他一些包埋树脂更大程度地掩盖抗原位点，并且它们不如粘性较小的包埋制剂那样能很好地渗透组织。还可能会显示出一些不寻常的特征，例如细胞器的收缩、染色后切片中的问题以及组织成分在包埋块和切片内的移动。本报告讨论了环氧树脂的一些特性。
