Silva Helen Aguiar Lemes da, Lima Gabriel Silva de, Boité Mariana Côrtes, Porrozzi Renato, Hueb Marcia, Damazo Amilcar Sabino
Pós-Graduação em Ciências da Saúde, Laboratório de Histologia, Universidade Federal de Mato Grosso, Cuiabá, Mato Grosso, Brazil.
Laboratório de Histologia, Faculdade de Medicina, Universidade Federal de Mato Grosso, Cuiabá, Mato Grosso, Brazil.
Rev Soc Bras Med Trop. 2015 Sep-Oct;48(5):560-7. doi: 10.1590/0037-8682-0183-2015.
The aim of this study was quantify annexin A1 expression in macrophages and cluster of differentiation 4 (CD4) + and cluster of differentiation 8 (CD8)+ T cells from the skin of patients with cutaneous leishmaniasis (n=55) and correlate with histopathological aspects.
Infecting species were identified by polymerase chain reaction-restriction fragment length polymorphism, and expression of annexin A1 was analyzed by immunofluorescence.
All patients (n = 55) were infected with Leishmania braziliensis . Annexin A1 was expressed more abundantly in CD163 + macrophages in infected skin (p < 0.0001) than in uninfected skin. In addition, macrophages in necrotic exudative reaction lesions expressed annexin A1 at higher levels than those observed in granulomatous (p < 0.01) and cellular lesions p < 0.05). This difference might be due to the need to clear both parasites and necrotic tissue from necrotic lesions. CD4 + cells in cellular lesions expressed annexin A1 more abundantly than did those in necrotic (p < 0.05) and granulomatous lesions (p < 0.01). Expression in CD8 + T cells followed the same trend. These differences might be due to the pervasiveness of lymphohistiocytic and plasmacytic infiltrate in cellular lesions.
Annexin A1 is differentially expressed in CD163 + macrophages and T cells depending on the histopathological features of Leishmania -infected skin, which might affect cell activation.
本研究的目的是量化皮肤利什曼病患者（n = 55）皮肤中巨噬细胞、分化簇4（CD4）+和分化簇8（CD8）+ T细胞中膜联蛋白A1的表达，并与组织病理学特征相关联。
通过聚合酶链反应-限制性片段长度多态性鉴定感染的物种，并通过免疫荧光分析膜联蛋白A1的表达。
所有患者（n = 55）均感染巴西利什曼原虫。与未感染皮肤相比，感染皮肤中的CD163 +巨噬细胞中膜联蛋白A1的表达更为丰富（p < 0.0001）。此外，坏死渗出反应性病变中的巨噬细胞表达膜联蛋白A1的水平高于肉芽肿性病变（p < 0.01）和细胞性病变（p < 0.05）中的巨噬细胞。这种差异可能是由于需要清除坏死病变中的寄生虫和坏死组织。细胞性病变中的CD4 +细胞比坏死性病变（p < 0.05）和肉芽肿性病变（p < 0.01）中的CD4 +细胞更丰富地表达膜联蛋白A1。CD8 + T细胞中的表达遵循相同趋势。这些差异可能是由于细胞性病变中淋巴细胞组织细胞和浆细胞浸润的普遍性。
根据利什曼原虫感染皮肤的组织病理学特征，膜联蛋白A1在CD163 +巨噬细胞和T细胞中差异表达，这可能影响细胞活化。
