Mattar Salim, Miranda Jorge, Pinzon Hernando, Tique Vanesa, Bolanos Amada, Aponte Jose, Arrieta German, Gonzalez Marco, Barrios Katerine, Contreras Hector, Alvarez Jaime, Aleman Ader
Universidad de Córdoba, Instituto de Investigaciones Biológicas del Trópico, Montería, Colombia.
J Infect Dev Ctries. 2015 Oct 29;9(10):1126-32. doi: 10.3855/jidc.6670.
The Caribbean area of Colombia has been severely affected by a Chikungunya virus (CHIKV) outbreak since 2014.
The study was carried out on 100 patients during a fever outbreak from August to September 2014 in two small rural villages in the northern Caribbean area of Colombia. The molecular assays performed by reverse transcription polymerase chain reaction (RT-PCR) on acute patient sera were collected within one to five days of the appearance of symptoms. Sequence analyses were carried out based on phylogenetic analyses of genes NS1 and E2. For serological assays, 49 (49%) patients at ≥ 6 days of disease onset were tested with NovaLisa Chikungunya IgG/IgM µ-capture enzyme-linked immunosorbent assay (ELISA).
The main signs or symptoms associated with Chikungunya infection were arthralgia of the lower limbs (96%), fever (91%), arthralgia of the upper limbs (85%), rash (64%), and headache (57%). Ninety-four percent (46/49) of patients were positive for either IgM or IgG; the remaining three (6%) patients were seronegative. Viral loads were detected in 25 patients. Based on phylogenetic analysis of NS1 and E2, the characterization of the Colombian CHIKV indicated that it was a strain closely related to the British Virgin Islands strain and to the Asian genotype.
This study shows the phylogenetic and clinical description of CHIKV in Colombia. The main symptoms shown were: arthralgia, fever, and rash. CHIKV sequences detected in Colombian patients were within the Asian genotype and closely related to the British Virgin Islands strain.
自2014年以来，哥伦比亚的加勒比地区受到基孔肯雅病毒（CHIKV）疫情的严重影响。
2014年8月至9月，在哥伦比亚加勒比地区北部的两个小乡村，对100名发热患者开展了此项研究。通过逆转录聚合酶链反应（RT-PCR）对急性患者血清进行分子检测，样本在症状出现后的1至5天内采集。基于NS1和E2基因的系统发育分析进行序列分析。对于血清学检测，在发病≥6天的49名（49%）患者中，采用NovaLisa基孔肯雅IgG/IgM μ捕获酶联免疫吸附测定（ELISA）进行检测。
与基孔肯雅感染相关的主要体征或症状为下肢关节痛（96%）、发热（91%）、上肢关节痛（85%）、皮疹（64%）和头痛（57%）。94%（46/49）的患者IgM或IgG呈阳性；其余3名（6%）患者血清学检测为阴性。在25名患者中检测到病毒载量。基于NS1和E2的系统发育分析，哥伦比亚CHIKV的特征表明它是一种与英属维尔京群岛毒株和亚洲基因型密切相关的毒株。
本研究展示了哥伦比亚CHIKV的系统发育和临床描述。主要症状为：关节痛、发热和皮疹。在哥伦比亚患者中检测到的CHIKV序列属于亚洲基因型，且与英属维尔京群岛毒株密切相关。
