Reddington Samuel C, Howarth Mark
Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
Curr Opin Chem Biol. 2015 Dec;29:94-9. doi: 10.1016/j.cbpa.2015.10.002. Epub 2015 Oct 30.
SpyTag is a short peptide that forms an isopeptide bond upon encountering its protein partner SpyCatcher. This covalent peptide interaction is a simple and powerful tool for bioconjugation and extending what protein architectures are accessible. Here we review the origin and mechanism of SpyTag/SpyCatcher, focusing on recent innovative applications. Ligation of targeting-antibody with antigen provided a simple route to vaccine generation. SpyRings, from head-to-tail cyclisation, gave major enhancements in enzyme resilience. Linking multiple SpyCatchers gave dendrimers for T-cell activation or Spy networks forming hydrogels for stem cell culture. Synthetic biology applications include integrating amyloid biomaterials with living bacteria, for irreversible derivatisation of biofilms with enzymes or nanoparticles. We also discuss further opportunities to apply and enhance SpyTag/SpyCatcher technology.
SpyTag是一种短肽，它在遇到其蛋白质伴侣SpyCatcher时会形成异肽键。这种共价肽相互作用是一种简单而强大的生物缀合工具，可扩展可获得的蛋白质结构类型。在此，我们回顾SpyTag/SpyCatcher的起源和作用机制，重点关注近期的创新应用。靶向抗体与抗原的连接为疫苗生产提供了一条简单途径。通过首尾环化形成的Spy环显著提高了酶的稳定性。连接多个SpyCatcher可得到用于激活T细胞的树枝状大分子，或形成用于干细胞培养的水凝胶的Spy网络。合成生物学应用包括将淀粉样生物材料与活细菌整合，用于用酶或纳米颗粒对生物膜进行不可逆衍生化。我们还讨论了应用和改进SpyTag/SpyCatcher技术的更多机会。
