Kang Heeyoung, Kim Haneul, Joung Yochan, Joh Kiseong
Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Gyeonggi 449-791, Republic of Korea.
Department of Biological Sciences, Inha University, Incheon 402-751, Republic of Korea.
Int J Syst Evol Microbiol. 2016 Jan;66(1):360-364. doi: 10.1099/ijsem.0.000720. Epub 2015 Oct 29.
A Gram-stain-negative, curved rod-shaped and non-pigmented strain, HME8277T, was isolated from surface seawater of the Yellow Sea in the Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain was related most closely to Reinekea blandensis MED297T (96.4 % 16S rRNA gene sequence similarity), Reinekea aestuarii IMCC4489T (96.3 %) and Reinekea marinisedimentorum KMM 3655T (95.8 %). The major fatty acids were summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c; 43.0 %), C16 : 0 (19.0 %) and summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c; 15.0 %). The DNA G+C content was 46.1 mol%. The predominant respiratory quinone was Q-8. The major polar lipids of strain HME8277T comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, four unidentified phospholipids and four unidentified lipids. On the basis of polyphasic analyses, strain HME8277T represents a novel species of the genus Reinekea, for which the name Reinekea marina sp. nov. is proposed. The type strain HME8277T ( = KACC 17315T = CECT 8288T). An emended description of the genus Reinekea is also provided.
从大韩民国黄海表层海水中分离出一株革兰氏染色阴性、弯曲杆状且无色素的菌株HME8277T。基于16S rRNA基因序列的系统发育分析表明，该新菌株与平淡雷内克氏菌MED297T（16S rRNA基因序列相似性为96.4%）、河口雷内克氏菌IMCC4489T（96.3%）和海洋沉积雷内克氏菌KMM 3655T（95.8%）关系最为密切。主要脂肪酸为总和特征3（包含C16:1ω7c和/或C16:1ω6c；43.0%）、C16:0（19.0%）和总和特征8（包含C18:1ω7c和/或C18:1ω6c；15.0%）。DNA G+C含量为46.1mol%。主要呼吸醌为Q-8。菌株HME8277T的主要极性脂类包括双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、四种未鉴定的磷脂和四种未鉴定的脂类。基于多相分析，菌株HME8277T代表雷内克氏菌属的一个新物种，为此提出名称海洋雷内克氏菌新种（Reinekea marina sp. nov.）。模式菌株为HME8277T（=KACC 17315T=CECT 8288T）。还提供了雷内克氏菌属的修订描述。
