Caralt M
Servei Cirurgia Hepatobiliopancreàtica i Trasplants, Hospital Universitari Vall Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.
Transplant Proc. 2015 Oct;47(8):2377-9. doi: 10.1016/j.transproceed.2015.08.029.
In recent years, the creation of new organs using bioengineering has been proposed as a possible solution to the shortage of organs for transplantation. After decellularization of livers, the remaining extracellular matrix can be used as scaffolds that can be reseeded with different kinds of cells. Mature hepatocytes and fetal liver cells have been used, showing viability, functionality, and differentiation into mature cells. After perfusion in a bioreactor, the seeded scaffold or liver organoid may be transplanted. However, viability of these transplanted scaffolds is poor owing to the formation of thrombosis. We analyzed the recent advances in decellularizing and recellularizing and the results after transplantation reported in the literature.
近年来，利用生物工程创造新器官已被提议作为解决移植器官短缺问题的一种可能方案。肝脏脱细胞后，剩余的细胞外基质可用作支架，可重新接种不同类型的细胞。已使用成熟肝细胞和胎儿肝细胞，显示出活力、功能以及向成熟细胞的分化能力。在生物反应器中灌注后，接种的支架或肝类器官可进行移植。然而，由于血栓形成，这些移植支架的存活率较低。我们分析了文献中报道的脱细胞和再细胞化的最新进展以及移植后的结果。
