Willemse Jorke, Lieshout Ruby, van der Laan Luc J W, Verstegen Monique M A
Dept. Surgery, Erasmus MC - Rotterdam Medical Center, Rotterdam, The Netherlands.
Dept. Surgery, Erasmus MC - Rotterdam Medical Center, Rotterdam, The Netherlands.
Best Pract Res Clin Gastroenterol. 2017 Apr;31(2):151-159. doi: 10.1016/j.bpg.2017.03.003. Epub 2017 Apr 12.
Due to the complex function and structure of the liver, resourceful solutions for treating end-stage liver disease are required. Currently, liver transplantation is the only curative therapeutic option. However, due to a worldwide donor shortage, researchers have been looking in other fields for alternative sources of transplantable liver tissue. Recent advances in our understanding of liver physiology, stem cell and matrix biology, have accelerated tissue engineering research. Most notable is the discovery of a culture system to grow liver-like organoids from human hepatic stem cells. The extensive expansion capacity of these stem cells has contributed greatly to the availability of hepatocyte-like cells for tissue engineering. In addition, new techniques are explored to obtain biological liver scaffolds from full size donor organs. This review summarizes these state-of-art techniques which may lay the groundwork towards re-creating transplantable tissue from autologous or allogenic stem cells in the coming decade.
由于肝脏功能和结构复杂，需要有足智多谋的解决方案来治疗终末期肝病。目前，肝移植是唯一的治愈性治疗选择。然而，由于全球供体短缺，研究人员一直在其他领域寻找可移植肝组织的替代来源。我们对肝脏生理学、干细胞和基质生物学认识的最新进展加速了组织工程研究。最值得注意的是发现了一种从人肝干细胞培养类肝器官的培养系统。这些干细胞的广泛扩增能力极大地促进了用于组织工程的类肝细胞的可得性。此外，人们正在探索新技术以从全尺寸供体器官获得生物肝支架。本综述总结了这些先进技术，它们可能为在未来十年从自体或同种异体干细胞重新创建可移植组织奠定基础。
