Barros M A P, Vasconcelos P R L, Souza C M, Andrade G M, Moraes M O, Costa P E G, Coelho G R, Garcia J H P
Department of Surgery, HUWC (University Hospital), Universidade Federal do Ceará, Fortaleza, Ceará, Brazil.
Department of Surgery, HUWC (University Hospital), Universidade Federal do Ceará, Fortaleza, Ceará, Brazil.
Transplant Proc. 2015 Oct;47(8):2478-82. doi: 10.1016/j.transproceed.2015.08.001.
Ischemia/reperfusion injury during liver transplantation can cause severe damage to the graft. The objective of this randomized, double-blind study was to evaluate the possible protective effects of L-alanyl-glutamine on the liver graft.
The sample included 33 patients from a liver transplantation service in Northeastern Brazil. Before cold ischemia, the patients received 50 g of L-alanyl-glutamine (treatment group) or saline (control group) through the portal vein. The graft was biopsied at the time of recovery, at the beginning of warm ischemia, and at the end of transplantation to determine malondialdehyde (MDA), heat-shock protein (Hsp)70, nuclear factor kappa-beta (NFkB), superoxide dismutase (SOD), and reduced glutathione (GSH) levels.
The blood parameters were similar in the two groups. In the treatment group, MDA did not increase at the beginning of cold ischemia and decreased at the end of transplantation. This phenomenon was not observed in the control group. GSH, SOD, Hsp70, and NFkB levels were similar in the two groups.
Our findings suggest that preconditioning with L-alanyl-glutamine attenuates the effects of ischemia/reperfusion-related oxidative stress and reduces lipid peroxidation in the grafts of liver transplantation patients.
肝移植期间的缺血/再灌注损伤可对移植物造成严重损害。这项随机、双盲研究的目的是评估L-丙氨酰-谷氨酰胺对肝移植物可能的保护作用。
样本包括来自巴西东北部一家肝移植服务机构的33例患者。在冷缺血前，患者通过门静脉接受50克L-丙氨酰-谷氨酰胺（治疗组）或生理盐水（对照组）。在恢复时、热缺血开始时和移植结束时对移植物进行活检，以测定丙二醛（MDA）、热休克蛋白（Hsp）70、核因子κB（NFkB）、超氧化物歧化酶（SOD）和还原型谷胱甘肽（GSH）水平。
两组的血液参数相似。在治疗组中，MDA在冷缺血开始时没有增加，在移植结束时下降。对照组未观察到这种现象。两组的GSH、SOD、Hsp70和NFkB水平相似。
我们的研究结果表明，L-丙氨酰-谷氨酰胺预处理可减轻缺血/再灌注相关氧化应激的影响，并减少肝移植患者移植物中的脂质过氧化。
