Boutet Emmanuel, Lieberherr Damien, Tognolli Michael, Schneider Michel, Bansal Parit, Bridge Alan J, Poux Sylvain, Bougueleret Lydie, Xenarios Ioannis
Swiss Institute of Bioinformatics, Centre Medical Universitaire, rue Michel Servet 1, CH-1211, Geneva 4, Switzerland.
University of Lausanne, CIG, Lausanne, 1015, Switzerland.
Methods Mol Biol. 2016;1374:23-54. doi: 10.1007/978-1-4939-3167-5_2.
The Universal Protein Resource (UniProt, http://www.uniprot.org ) consortium is an initiative of the SIB Swiss Institute of Bioinformatics (SIB), the European Bioinformatics Institute (EBI) and the Protein Information Resource (PIR) to provide the scientific community with a central resource for protein sequences and functional information. The UniProt consortium maintains the UniProt KnowledgeBase (UniProtKB), updated every 4 weeks, and several supplementary databases including the UniProt Reference Clusters (UniRef) and the UniProt Archive (UniParc).The Swiss-Prot section of the UniProt KnowledgeBase (UniProtKB/Swiss-Prot) contains publicly available expertly manually annotated protein sequences obtained from a broad spectrum of organisms. Plant protein entries are produced in the frame of the Plant Proteome Annotation Program (PPAP), with an emphasis on characterized proteins of Arabidopsis thaliana and Oryza sativa. High level annotations provided by UniProtKB/Swiss-Prot are widely used to predict annotation of newly available proteins through automatic pipelines.The purpose of this chapter is to present a guided tour of a UniProtKB/Swiss-Prot entry. We will also present some of the tools and databases that are linked to each entry.
通用蛋白质资源（UniProt，http://www.uniprot.org ）联盟是由瑞士生物信息学研究所（SIB）、欧洲生物信息学研究所（EBI）和蛋白质信息资源（PIR）发起的一项计划，旨在为科学界提供一个蛋白质序列和功能信息的核心资源库。UniProt联盟维护着每4周更新一次的UniProt知识库（UniProtKB）以及几个补充数据库，包括UniProt参考簇（UniRef）和UniProt存档（UniParc）。UniProt知识库（UniProtKB/Swiss-Prot）的Swiss-Prot部分包含从广泛生物体中获得的公开可用的、经过专家手动注释的蛋白质序列。植物蛋白质条目是在植物蛋白质组注释计划（PPAP）框架内生成的，重点是拟南芥和水稻中已鉴定的蛋白质。UniProtKB/Swiss-Prot提供的高级注释被广泛用于通过自动流程预测新获得蛋白质的注释。本章的目的是介绍一次对UniProtKB/Swiss-Prot条目的指南式浏览。我们还将介绍一些与每个条目相关联的工具和数据库。
