Demitri Christian, Raucci Maria Grazia, Giuri Antonella, De Benedictis Vincenzo Maria, Giugliano Daniela, Calcagnile Paola, Sannino Alessandro, Ambrosio Luigi
Department of Engineering for Innovation, University of Salento, via Monteroni, Km 1, Lecce, 73100, Italy.
Institute of Polymers, Composites and Biomaterials (IPCB), National Research Council of Italy Mostra D'oltremare Pad.20, Viale Kennedy 54, Naples, 80125, Italy.
J Biomed Mater Res A. 2016 Mar;104(3):726-733. doi: 10.1002/jbm.a.35611. Epub 2015 Nov 23.
Physical foaming combined with microwave-induced curing was used in this study to develop an innovative device for bone tissue regeneration. In the first step of the process, a stable physical foaming was induced using a surfactant (i.e. pluronic) as blowing agent of a homogeneous blend of Sodium salt of carboxymethylcellulose (CMCNa) and polyethylene glycol diacrylate (PEGDA700) solution. In the second step, the porous structure of the scaffold was chemically stabilized by radical polymerization induced by a homogeneous rapid heating of the sample in a microwave reactor. In this step 2,2-Azobis[2-(2-imidazolin-2 yl)propane]Dihydrochloride was used as thermoinitiator (TI). CMCNa and PEGDA were mixed with different blends to correlate the properties of final product with the composition. The chemical properties of each sample were evaluated by spectroscopy analysis ATR-IR (before and after curing) in order to maximize reaction yield, and optimize kinetic parameters (i.e. time curing, microwave power). The stability of the materials was evaluated in vitro by degradation test in Phosphate Buffered Saline. Biological analyses were performed to evaluate the effect of scaffold materials on cellular behavior in terms of proliferation and early osteogenic differentiation of human Mesenchymal Stem Cells. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 726-733, 2016.
本研究采用物理发泡与微波诱导固化相结合的方法，开发一种用于骨组织再生的创新装置。在该过程的第一步，使用表面活性剂（即普朗尼克）作为羧甲基纤维素钠（CMCNa）和聚乙二醇二丙烯酸酯（PEGDA700）溶液均匀混合物的发泡剂，诱导产生稳定的物理发泡。在第二步中，通过在微波反应器中对样品进行均匀快速加热引发自由基聚合反应，使支架的多孔结构化学稳定。在这一步中，使用2,2-偶氮二[2-(2-咪唑啉-2-基)丙烷]二盐酸盐作为热引发剂（TI）。将CMCNa和PEGDA与不同的混合物混合，以关联最终产品的性能与组成。通过ATR-IR光谱分析（固化前后）评估每个样品的化学性质，以最大化反应产率并优化动力学参数（即固化时间、微波功率）。通过在磷酸盐缓冲盐水中的降解试验对材料的稳定性进行体外评估。进行生物学分析，以评估支架材料对人骨髓间充质干细胞增殖和早期成骨分化方面细胞行为的影响。© 2015威利期刊公司。《生物医学材料研究杂志》A部分：第104A卷：726 - 733页，2016年。
