骨关节炎大鼠内侧半月板撕裂模型中软骨下骨微结构、矿化及力学性能的动态变化
Dynamic Alterations in Microarchitecture, Mineralization and Mechanical Property of Subchondral Bone in Rat Medial Meniscal Tear Model of Osteoarthritis.
作者信息
Yu De-Gang, Nie Shao-Bo, Liu Feng-Xiang, Wu Chuan-Long, Tian Bo, Wang Wen-Gang, Wang Xiao-Qing, Zhu Zhen-An, Mao Yuan-Qing
机构信息
Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
出版信息
Chin Med J (Engl). 2015 Nov 5;128(21):2879-86. doi: 10.4103/0366-6999.168045.
BACKGROUND
The properties of subchondral bone influence the integrity of articular cartilage in the pathogenesis of osteoarthritis (OA). However, the characteristics of subchondral bone alterations remain unresolved. The present study aimed to observe the dynamic alterations in the microarchitecture, mineralization, and mechanical properties of subchondral bone during the progression of OA.
METHODS
A medial meniscal tear (MMT) operation was performed in 128 adult Sprague Dawley rats to induce OA. At 2, 4, 8, and 12 weeks following the MMT operation, cartilage degeneration was evaluated using toluidine blue O staining, whereas changes in the microarchitecture indices and tissue mineral density (TMD), mineral-to-collagen ratio, and intrinsic mechanical properties of subchondral bone plates (BPs) and trabecular bones (Tbs) were measured using micro-computed tomography scanning, confocal Raman microspectroscopy and nanoindentation testing, respectively.
RESULTS
Cartilage degeneration occurred and worsened progressively from 2 to 12 weeks after OA induction. Microarchitecture analysis revealed that the subchondral bone shifted from bone resorption early (reduced trabecular BV/TV, trabecular number, connectivity density and trabecular thickness [Tb.Th], and increased trabecular spacing (Tb.Sp) at 2 and 4 weeks) to bone accretion late (increased BV/TV, Tb.Th and thickness of subchondral bone plate, and reduced Tb.Sp at 8 and 12 weeks). The TMD of both the BP and Tb displayed no significant changes at 2 and 4 weeks but decreased at 8 and 12 weeks. The mineral-to-collagen ratio showed a significant decrease from 4 weeks for the Tb and from 8 weeks for the BP after OA induction. Both the elastic modulus and hardness of the Tb showed a significant decrease from 4 weeks after OA induction. The BP showed a significant decrease in its elastic modulus from 8 weeks and its hardness from 4 weeks.
CONCLUSION
The microarchitecture, mineralization and mechanical properties of subchondral bone changed in a time-dependent manner as OA progressed.
背景
在骨关节炎（OA）发病机制中，软骨下骨的特性会影响关节软骨的完整性。然而，软骨下骨改变的特征仍未明确。本研究旨在观察OA进展过程中软骨下骨的微观结构、矿化及力学性能的动态变化。
方法
对128只成年Sprague Dawley大鼠进行内侧半月板撕裂（MMT）手术以诱导OA。在MMT手术后2、4、8和12周，使用甲苯胺蓝O染色评估软骨退变，而分别使用微型计算机断层扫描、共聚焦拉曼光谱和纳米压痕测试测量软骨下骨板（BPs）和小梁骨（Tbs）的微观结构指数、组织矿物质密度（TMD）、矿物质与胶原蛋白比率以及固有力学性能的变化。
结果
OA诱导后2至12周，软骨退变逐渐发生并加重。微观结构分析显示，软骨下骨从早期的骨吸收（2周和4周时小梁骨体积分数、小梁数量、连接密度和小梁厚度[Tb.Th]降低，小梁间距[Tb.Sp]增加）转变为后期的骨增生（8周和12周时骨体积分数、Tb.Th和软骨下骨板厚度增加，Tb.Sp降低）。BP和Tb的TMD在2周和4周时无显著变化，但在8周和12周时降低。OA诱导后，Tb的矿物质与胶原蛋白比率从4周开始显著降低，BP从8周开始显著降低。Tb的弹性模量和硬度从OA诱导后4周开始显著降低。BP的弹性模量从8周开始显著降低，硬度从4周开始显著降低。
结论
随着OA的进展软骨下骨的微观结构、矿化和力学性能呈时间依赖性变化。
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