Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, 109 Xue Yuan Xi Road, Wenzhou, Zhejiang, 325000, China.
Department of Orthopaedics, Yongjia County People's Hospital, 37 Yong Zhong Road, Shang Tang Town, Yongjia County, Zhejiang, 325100, China.
Inflammation. 2018 Mar;41(2):390-399. doi: 10.1007/s10753-017-0694-2.
Osteoarthritis (OA) is a chronic degenerative joint disease that is principally characterized by progressive joint dysfunction and cartilage degradation. Inflammation and apoptosis play critical roles in the progression of OA. Geniposide (GPO), one of the principal components of the fruit of Gardenia jasminoides Ellis, has been reported to have anti-inflammatory and other pharmacological effects. In this study, we performed in vitro experiments on rat chondrocytes to examine the therapeutic effects of GPO on OA and investigated its effects in vivo in a rat model of OA induced by medial meniscal tear (MMT). The results suggest that GPO can inhibit the expression of INOS, COX-2, and MMP-13 in vitro, and promote the expression of collagen II in rat chondrocytes stimulated with interleukin-1β (IL-1β). In addition, we also found that GPO can inhibit the expression of pro-apoptotic proteins such as Bax, Cyto-c, and C-caspase3 and increase the expression of the anti-apoptotic protein Bcl-2. These changes may be related to GPO-induced inhibition of the IL-1β-induced activation of the PI3K/Akt/NF-κB signaling pathway. In vivo, we also found that GPO can limit the development of OA in a rat model. Taken together, the above results indicate that GPO has potential therapeutic value for treating OA.
骨关节炎（OA）是一种慢性退行性关节疾病，主要表现为进行性关节功能障碍和软骨降解。炎症和细胞凋亡在 OA 的进展中起着关键作用。栀子苷（GPO）是栀子果实的主要成分之一，据报道具有抗炎等药理作用。本研究在大鼠软骨细胞体外实验中观察了 GPO 对 OA 的治疗作用，并在半月板内侧撕裂（MMT）诱导的大鼠 OA 模型中进行了体内研究。结果表明，GPO 能抑制体外白细胞介素-1β（IL-1β）刺激的大鼠软骨细胞中 INOS、COX-2 和 MMP-13 的表达，并促进胶原 II 的表达。此外，我们还发现 GPO 能抑制促凋亡蛋白 Bax、Cyto-c 和 C-caspase3 的表达，增加抗凋亡蛋白 Bcl-2 的表达。这些变化可能与 GPO 抑制 IL-1β诱导的 PI3K/Akt/NF-κB 信号通路激活有关。在体内，我们还发现 GPO 能限制大鼠模型中 OA 的发展。综上所述，这些结果表明 GPO 对治疗 OA 具有潜在的治疗价值。
