从9号染色体三体镶嵌胚胎建立人胚胎干细胞系。

Establishment of human-embryonic-stem-cell line from mosaic trisomy 9 embryo.

作者信息

Huang Boxian, Jiang Chunyan, Chen Aiqin, Cui Yugui, Xie Jiazi, Shen Jiandong, Chen Juan, Cai Lingbo, Liao Tingting, Ning Song, Jiang Shi-Wen, Fan Guoping, Qin Lianju, Liu Jiayin

机构信息

State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China; School of Life Science and Technology, China Pharmaceutical University, Nanjing 210038, China.

State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.

出版信息

Taiwan J Obstet Gynecol. 2015 Oct;54(5):505-11. doi: 10.1016/j.tjog.2015.08.003.

DOI:10.1016/j.tjog.2015.08.003

PMID:26522100

Abstract

OBJECTIVE

Human-embryonic-stem-cell (hESC) lines derived from chromosomally or genetically abnormal embryos obtained following preimplantation genetic diagnosis are valuable in investigating genetic disorders.

MATERIALS AND METHODS

In this study, a new hESC line, Center of Clinical Reproductive Medicine 8 (CCRM8) was established by isolation, culture, and passaging of the inner cell mass of mosaic trisomy 9 embryos.

RESULTS

A karyotype analysis showed that the hESC line possessed a euploid (46 chromosomes). The undifferentiated hESCs exhibited long-term proliferation capacity and expressed typical markers of OCT4, TRA-1-60, and TRA-1-81. In vitro embryoid-body (EB) formation, differentiation, and in vivo teratoma production confirmed the pluripotency of the hESC line. The data represented here are the first detailed report on the characterization and differentiation of one Chinese hESC line generated from mosaic trisomy 9 embryos.

CONCLUSION

Our study showed that chromosomally aberrant embryos could generate a normal hESC line, which would be useful in investigating gene function and embryo development.

摘要

目的

从植入前基因诊断后获得的染色体或基因异常胚胎中衍生出的人胚胎干细胞（hESC）系，在研究遗传疾病方面具有重要价值。

材料与方法

在本研究中，通过分离、培养和传代嵌合型9号三体胚胎的内细胞团，建立了一个新的hESC系，即临床生殖医学中心8（CCRM8）。

结果

核型分析显示该hESC系具有整倍体（46条染色体）。未分化的hESCs表现出长期增殖能力，并表达OCT4、TRA-1-60和TRA-1-81等典型标志物。体外胚状体（EB）形成、分化以及体内畸胎瘤生成证实了该hESC系的多能性。此处呈现的数据是关于从嵌合型9号三体胚胎产生的一个中国hESC系的特征和分化的首次详细报告。