Nikooie Rohollah, Samaneh Sajadian
Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Shahid Bahonar University of Kerman, Kerman, Iran; Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Shahid Bahonar University of Kerman, Kerman, Iran.
Mol Cell Endocrinol. 2016 Jan 5;419:244-51. doi: 10.1016/j.mce.2015.10.024. Epub 2015 Oct 29.
The mechanism regulating the utilization of intramuscular triacylglycerol (IMTG) during high-intensity interval training (HIIT) and post-exercise recovery period remains elusive. In this study, the acute and long-term effects of HIIT on transforming growth factor beta 1 (TGF-β1) abundance in rat skeletal muscle and role of lactate and TGF-β1 in IMTG lipolysis during post-exercise recovery period were examined. TGF-β1 and Adipose triacylglycerol lipase (ATGL) abundance as well as total lipase activity in the gastrocnemius muscle significantly increased to a maximum value 10 h after acute bout of HIIT. Inhibition of TGF-β1 signaling by intramuscular injection of SB431542 30 min prior to the acute exercise attenuated ATGL abundance and total lipase activity in the gastrocnemius muscle in response to acute exercise. Intramuscular acute injection of lactate increased TGF-β1 and ATGL abundance in the gastrocnemius muscle and there were a significant increase in Muscle TGF-β1 and ATGL abundance after 5 weeks of HIIT/lactate treatment. These results indicate that exercise-induced lactate accumulation regulates intramuscular triglyceride metabolism via transforming growth factor-β1 mediated pathways during post-exercise recovery from strenuous exercise.
高强度间歇训练（HIIT）及运动后恢复期肌肉内三酰甘油（IMTG）利用的调节机制仍不清楚。本研究检测了HIIT对大鼠骨骼肌中转化生长因子β1（TGF-β1）丰度的急性和长期影响，以及乳酸和TGF-β1在运动后恢复期IMTG脂解中的作用。急性HIIT发作后10小时，腓肠肌中TGF-β1和脂肪三酰甘油脂肪酶（ATGL）的丰度以及总脂肪酶活性显著增加至最大值。在急性运动前30分钟通过肌肉注射SB431542抑制TGF-β1信号传导，可减弱急性运动后腓肠肌中ATGL的丰度和总脂肪酶活性。肌肉内急性注射乳酸可增加腓肠肌中TGF-β1和ATGL的丰度，并且在HIIT/乳酸治疗5周后，肌肉中TGF-β1和ATGL的丰度显著增加。这些结果表明，在剧烈运动后的恢复期中，运动诱导的乳酸积累通过转化生长因子-β1介导的途径调节肌肉内甘油三酯代谢。
