四种海洋来源真菌提取物在七种人类癌细胞系中作为抗增殖和诱导细胞死亡剂的潜力。
Potential of four marine-derived fungi extracts as anti-proliferative and cell death-inducing agents in seven human cancer cell lines.
作者信息
Ramos Alice Abreu, Prata-Sena Maria, Castro-Carvalho Bruno, Dethoup Tida, Buttachon Suradet, Kijjoa Anake, Rocha Eduardo
机构信息
Interdisciplinary Center for Marine and Environmental Research (CIIMAR), CIMAR Associate Laboratory (CIMAR LA), University of Porto (U. Porto), Rua dos Bragas, nº 289, 4050-123 Porto, Portugal.
Interdisciplinary Center for Marine and Environmental Research (CIIMAR), CIMAR Associate Laboratory (CIMAR LA), University of Porto (U. Porto), Rua dos Bragas, nº 289, 4050-123 Porto, Portugal; ICBAS - Institute of Biomedical Sciences Abel Salazar, University of Porto (U. Porto), Rua de Jorge Viterbo Ferreira, nº 228, 4050-313 Porto, Portugal.
出版信息
Asian Pac J Trop Med. 2015 Oct;8(10):798-806. doi: 10.1016/j.apjtm.2015.09.005. Epub 2015 Sep 25.
OBJECTIVE
To evaluate the in vitro anticancer activity of crude ethyl acetate extracts of the culture of four marine-derived fungi Aspergillus similanensis KUFA 0013 (E1), Neosartorya paulistensis KUFC 7897 (E2), Neosartorya siamensis KUFA 0017 (E4) and Talaromyces trachyspermus KUFC 0021 (E3) on a panel of seven human cancer cell lines.
METHODS
Effects on cell proliferation, induction of DNA damage and cell death were assessed by MTT and clonogenic assays, comet assay and nuclear condensation assay, respectively.
RESULTS
The proliferation of HepG2, HCT116 and A375 cells decreased after incubation with the extracts E2 and E4. The anti-proliferative effect was confirmed by morphologic alterations and by clonogenic assay. Both extracts also induced cell death in HepG2 and HCT116 cells. Doxorubicin was used as a positive control and showed in vitro anticancer activity.
CONCLUSIONS
This study demonstrated, for the first time, that extracts of Neosartorya paulistensis and Neosartorya siamensis have selective anti-proliferative and cell death activities in HepG2, HCT16 and A375 cells. The bioactivity of these extracts suggests a potential for biotechnological applications and substantiates that both should be further considered for the elucidation of the molecular targets and signal transduction pathways involved.
目的
评估四种海洋来源真菌——相似曲霉KUFA 0013（E1）、圣保罗新萨托菌KUFC 7897（E2）、暹罗新萨托菌KUFA 0017（E4）和粗糙孢穗霉KUFC 0021（E3）培养物的粗乙酸乙酯提取物对一组七种人类癌细胞系的体外抗癌活性。
方法
分别通过MTT和克隆形成试验、彗星试验和核浓缩试验评估对细胞增殖、DNA损伤诱导和细胞死亡的影响。
结果
用提取物E2和E4孵育后，HepG2、HCT116和A375细胞的增殖减少。形态学改变和克隆形成试验证实了抗增殖作用。两种提取物还诱导了HepG2和HCT116细胞的死亡。阿霉素用作阳性对照，显示出体外抗癌活性。
结论
本研究首次证明，圣保罗新萨托菌和暹罗新萨托菌的提取物对HepG2、HCT16和A375细胞具有选择性抗增殖和细胞死亡活性。这些提取物的生物活性表明其在生物技术应用方面具有潜力，并证实两者都应进一步用于阐明所涉及的分子靶点和信号转导途径。
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