Gao Meng, Wu Fuling, Li Yingying, Shi Tao, Tian Lijun, Han Tingting, Wang Haiying
Department of Pediatrics, College of Clinical Medicine, Binzhou Medical University, Binzhou 256003, China.
Department of Pediatrics, Affiliated Hospital of Binzhou Medical University, Binzhou 256003, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2015 Nov;31(11):1492-6.
To study the level of retinoic acid receptor-related orphan receptor γt (RORγt) in rat lung tissues of bronchiolitis caused by respiratory syncytial virus (RSV) and its implication.
The rats were randomly divided into normal group and bronchiolitis group. After the model of bronchiolitis was established successfully by nasal dripping, the pathological changes of lung tissues were detected by HE staining; the plasma levels of interleukin 23 (IL-23), IL-17 were detected by ELISA; the level of RORγt mRNA in lung tissues and peripheral blood mononuclear cells (PBMCs) were detected by real-time quantitative PCR; the level of RORγt protein in lung tissues was examined by Western blotting.
Compared with the normal group, the rats with bronchiolitis presented with pulmonary interstitial hyperemia and edema, more inflammatory cell infiltration, wider alveolar septa and bronchial collapse and deformation. Compared with the normal group, the level of RORγt mRNA in the lung tissues and PBMCs increased in rats with bronchiolitis. The level of RORγt protein in lung tissues and the plasma levels of IL-23 and IL -17 were higher in rats with bronchiolitis than in normal rats.
The level of RORγt was elevated in the lung tissues of rats with RSV-induced bronchiolitis.
研究呼吸道合胞病毒（RSV）所致细支气管炎大鼠肺组织中维甲酸受体相关孤儿受体γt（RORγt）的水平及其意义。
将大鼠随机分为正常组和细支气管炎组。滴鼻成功建立细支气管炎模型后，采用HE染色检测肺组织病理变化；采用酶联免疫吸附测定（ELISA）法检测血浆白细胞介素23（IL-23）、IL-17水平；采用实时定量聚合酶链反应（PCR）检测肺组织及外周血单个核细胞（PBMCs）中RORγt mRNA水平；采用蛋白质免疫印迹法检测肺组织中RORγt蛋白水平。
与正常组相比，细支气管炎大鼠出现肺间质充血、水肿，炎症细胞浸润增多，肺泡间隔增宽，支气管塌陷变形。与正常组相比，细支气管炎大鼠肺组织及PBMCs中RORγt mRNA水平升高。细支气管炎大鼠肺组织中RORγt蛋白水平及血浆中IL-23、IL-17水平高于正常大鼠。
RSV诱导的细支气管炎大鼠肺组织中RORγt水平升高。
