Moioli B, Pilla F, Ciani E
J Anim Sci. 2015 Oct;93(10):4660-9. doi: 10.2527/jas.2015-9389.
Identification of genomic regions that have been targets of selection for phenotypic traits is one of the most challenging applications of dense marker panels in animal genetics. In this study, a genomewide scan using approximately 50,000 SNP was performed in an attempt to identify genomic regions associated with fat deposition in sheep, the importance of this not only being limited to livestock facing future climate changes but also for elucidating the physiology of lipid metabolism. The genotyping results obtained with the Ovine SNP50K BeadChip in 2 fat tail breeds were compared with those obtained in 13 thin tail breeds. Direct sequencing of the annotated genes located in proximity to the markers with opposite allele frequency in thin tail vs. fat tail sheep gave additional SNP of interest. To further confirm the results of the genomewide scan, we genotyped the SNP within these genes in the 2 groups of sheep. A missense mutation in the gene, with different allele frequency in the 2 groups, was detected. The results indicated and as the most probable genes involved in the fat tail phenotype.
识别作为表型性状选择目标的基因组区域是密集标记面板在动物遗传学中最具挑战性的应用之一。在本研究中，使用约50,000个单核苷酸多态性（SNP）进行全基因组扫描，旨在识别与绵羊脂肪沉积相关的基因组区域，其重要性不仅限于面临未来气候变化的家畜，还在于阐明脂质代谢的生理学。将使用绵羊SNP50K芯片在2个肥尾品种中获得的基因分型结果与在13个瘦尾品种中获得的结果进行比较。对瘦尾羊与肥尾羊中等位基因频率相反的标记附近的注释基因进行直接测序，得到了其他感兴趣的SNP。为了进一步确认全基因组扫描的结果，我们对这两组绵羊中这些基因内的SNP进行了基因分型。检测到该基因中的一个错义突变，在两组中的等位基因频率不同。结果表明，该基因是参与肥尾表型的最可能基因。
