[中国南京汉族献血者中HLA-Cw及其相应杀伤细胞免疫球蛋白样受体（KIR）的频率]

[Frequency of HLA-Cw and Its Corresponding Killer Cell Immunoglobulin-like Receptors (KIR) among Blood Donors in Chinese Nanjing Han Ethnic Group].

作者信息

Lin Hong, Ma Ling, Xue Min, Liu Yan-Chun

机构信息

Blood Center of Jiangsu Province, Nanjing 210042, Jiangsu Province, China.

Blood Center of Jiangsu Province, Nanjing 210042, Jiangsu Province, China. E-mail:

出版信息

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2015 Oct;23(5):1469-73. doi: 10.7534/j.issn.1009-2137.2015.05.047.

DOI:10.7534/j.issn.1009-2137.2015.05.047

PMID:26524059

Abstract

OBJECTIVE

To investigate the frequency of HLA-Cw and its KIR2D genotypes in Han blood donor population in Chinese Nanjing area and to analyze the match and distinguish modes of them so as to provide the basis for further studying their roles in incidence and development of disease.

METHODS

The PCR-SSP was used to genotyping of HLA-Cw and KIR2D for 241 Han blood donors in Jiangsu Provincial blood center; according to distingush modes of HLA-Cw and KIR2D genes, the distingushed results of HLA-Cw and corresponding activating or inhibitory KIR2D receptors of individuals were analyzed.

RESULTS

The frequency of HLA-C1 expression in donor population of Nanjing area was 76.35% which was much higher than that of HLA-C2 expression (23.65%); the expression C1/C2 alleles was accorded with Handy-weinberg balance. The expression frequencies of 5 KIR2Ds (L1, L2, L3, S1 and S2) matched to HLA-Cw were 97.93%, 29.05%, 98.34%, 29.05% and 21.16%, respectively. The match of HLA-C1/C2 to 2DL1⁺/2DL2⁻/2DL3⁺/2DS1⁻/2DS2⁻ was predominated (75/241). Couclusion: The polymorphism data of HLA-Cw and 5 KIR2Ds from blood donors in Chinese Nanjing area has been provided in this study. The match analysis found that the expression of inhibitory HLA-Cw-KIR is higher than that of activated HLA-Cw-KIR, suggesting that the HLA-Cw/KIR2D combination is characterized by preponderance of inhibitory signal pathway.

摘要

目的

研究中国南京地区汉族献血人群中HLA-Cw及其KIR2D基因的频率，并分析它们的匹配及区分模式，为进一步研究其在疾病发生发展中的作用提供依据。

方法

采用聚合酶链反应-序列特异性引物（PCR-SSP）技术对江苏省血液中心241名汉族献血者的HLA-Cw和KIR2D进行基因分型；根据HLA-Cw和KIR2D基因的区分模式，分析个体HLA-Cw的区分结果及其相应的激活或抑制性KIR2D受体。

结果

南京地区献血人群中HLA-C1的表达频率为76.35%，远高于HLA-C2的表达频率（23.65%）；C1/C2等位基因的表达符合哈迪-温伯格平衡。与HLA-Cw匹配的5种KIR2D（L1、L2、L3、S1和S2）的表达频率分别为97.93%、29.05%、98.34%、29.05%和21.16%。HLA-C1/C2与2DL1⁺/2DL2⁻/2DL3⁺/2DS1⁻/2DS2⁻的匹配占主导（75/241）。结论：本研究提供了中国南京地区献血者HLA-Cw和5种KIR2D的多态性数据。匹配分析发现，抑制性HLA-Cw-KIR的表达高于激活性HLA-Cw-KIR，提示HLA-Cw/KIR2D组合以抑制性信号通路占优势为特征。