Jiang Li-xin, Xu Lian-hong, Qi Chuan-ping, Wang Yong-fang
Department of Clinical Laboratory, Jiangsu University, Changzhou, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2012 Aug;28(8):863-5.
To investigate the distribution of killer cell immunoglobulin-like receptor (KIR) and its specific ligand human leukocyte antigen (HLA) in Jiangsu Han population.
173 samples from unrelated healthy individuals of Jiangsu Han population were genotyped and observed for KIR, HLA-Cw and HLA-Bw4 using a SYBR Green I real-time PCR and PCR-SSP method, respectively. The number and type of KIR/HLA pairs inherited in each individual were analyzed.
In Jiangsu Han population, all four inhibitory KIR (2DL1, 2DL2/3, 3DL1 and 3DL2) that recognize the classical HLA class I molecules HLA-A, -B and -C were present in >92% of the study group. Frequencies of 2DL2/HLA-C1, 2DL3/HLA-C1, 2DL1/HLA-C2 and 3DL1/Bw4 were 0.243, 0.971, 0.457 and 0.590, respectively; frequencies of 2DS1/HLA-C2 and 2DS2/HLA-C1 were 0.162 and 0.231, respectively. 54.3% of the cases expressed KIR2DL1 without HLA-C1, 32.9% inherited 3DL1 without HLA-Bw4 and 5.8% expressed HLA-Bw4 without 3DL1. 27.7% of the individuals had three iKIR/HLA pairs, 26% carried two iKIR/HLA pairs, and 25.4% inherited a single iKIR/HLA pair and no one was deficient in all three iKIR/HLA pairs.
There was disparity between KIR receptor and HLA ligand in Jiangsu Han population. Inhibitory KIR/HLA pair frequency was higher than stimulatory one. About 1/4 of the study group expressed a single iKIR/HLA pair alone.
研究杀伤细胞免疫球蛋白样受体（KIR）及其特异性配体人类白细胞抗原（HLA）在江苏汉族人群中的分布情况。
采用SYBR Green I实时荧光定量PCR和PCR-SSP方法，分别对173例江苏汉族无关健康个体的样本进行基因分型，观察KIR、HLA-Cw和HLA-Bw4。分析每个个体中遗传的KIR/HLA对的数量和类型。
在江苏汉族人群中，识别经典HLA I类分子HLA-A、-B和-C的所有四种抑制性KIR（2DL1、2DL2/3、3DL1和3DL2）在>92%的研究组中存在。2DL2/HLA-C1、2DL3/HLA-C1、2DL1/HLA-C2和3DL1/Bw4的频率分别为0.243、0.971、0.457和0.590；2DS1/HLA-C2和2DS2/HLA-C1的频率分别为0.162和0.231。54.3%的病例表达KIR2DL1但无HLA-C1，32.9%遗传3DL1但无HLA-Bw4，5.8%表达HLA-Bw4但无3DL1。27.7%的个体有三对抑制性KIR/HLA，26%携带两对抑制性KIR/HLA，25.4%遗传一对抑制性KIR/HLA，且没有个体所有三对抑制性KIR/HLA均缺失。
江苏汉族人群中KIR受体与HLA配体之间存在差异。抑制性KIR/HLA对的频率高于刺激性KIR/HLA对。约1/4的研究组个体单独表达一对抑制性KIR/HLA。
