Tian Qing, Ong Say Kee, Xie Xuehui, Li Fang, Zhu Yanbin, Wang Feng Rui, Yang Bo
Department of Environmental Science and Engineering, DongHua University, 2999 Shanghai North People's Road, 201620, PR China.
Department of Civil, Construction, and Environmental Engineering, Iowa State University, Iowa 50011, USA.
Chemosphere. 2016 Feb;144:1797-806. doi: 10.1016/j.chemosphere.2015.10.072. Epub 2015 Nov 11.
The operation of an alternating anaerobic/aerobic biofilter (AABF), treating synthetic wastewater, was modified to enhance recovery of phosphorus (P). The AABF was periodically fed with an additional carbon source during the anaerobic phase to force the release of biofilm-sequestered P which was then harvested and recovered. A maximum of 48% of the total influent P was found to be released in the solution for recovery. Upon implementation of periodic P bio-sequestering and P harvesting, the predominant bacterial communities changed from β-Proteobacteria to γ-Proteobacteria groups. The genus Pseudomonas of γ-Proteobacteria was found to enrich greatly with 98% dominance. Dense intracellular poly-P granules were found within the cells of the biofilm, confirming the presence of P accumulating organisms (PAOs). Periodic addition of a carbon source to the AABF coupled with intracellular P reduction during the anaerobic phase most probably exerted environmental stress in the selection of Pseudomonas PAOs over PAOs of other phylogenic types. Results of the study provided operational information on the selection of certain microbial communities for P removal and recovery. This information can be used to further advance P recovery in biofilm systems such as the AABFs.
对处理合成废水的交替厌氧/好氧生物滤池（AABF）的运行进行了改进，以提高磷（P）的回收。在厌氧阶段，向AABF定期添加额外的碳源，促使生物膜中封存的磷释放出来，随后进行收集和回收。发现溶液中最多可释放出48%的进水总磷以供回收。在实施定期的磷生物封存和磷收集后，主要细菌群落从β-变形菌纲转变为γ-变形菌纲。发现γ-变形菌纲的假单胞菌属大量富集，占比98%。在生物膜细胞内发现了密集的细胞内聚磷颗粒，证实了聚磷菌（PAO）的存在。在厌氧阶段向AABF定期添加碳源并伴随着细胞内磷的减少，很可能在选择假单胞菌PAO而非其他系统发育类型的PAO时施加了环境压力。该研究结果提供了有关选择特定微生物群落进行磷去除和回收的运行信息。这些信息可用于进一步推进生物膜系统（如AABF）中的磷回收。
