Meng Xuan, Pan Yang, Zhang Hao, Liao Xuan-Hong, Xu Lin-Jian, Femg Xin, Shan Jie
School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China.
Environment Biotechnology Research Institute, Suzhou University of Science and Technology, Suzhou 215009, China.
Huan Jing Ke Xue. 2018 Jun 8;39(6):2802-2809. doi: 10.13227/j.hjkx.201710048.
The purpose of this study was to develop a method to remove and recover high concentration phosphate solutions from wastewater. An experiment was carried out to cultivate and enrich phosphorus accumulating organisms (PAOs) in the biofilm with nylon as the biological carrier using artificial water distribution. Microflora morphology, species diversity, and the genetic relationship of biofilm during the process of biofilm domestication were studied by scanning electron microscopy (SEM) and MiSeq high-throughput sequencing. In addition, the feasibility of recycling a high concentration of phosphate in the conventional biofilm within a short time was validated. The membrane was hung in the biological carrier when the reactor was operated for 10 d. After the hanging of the film succeeded, the effluent COD was below 50 mg·L, the effluent phosphorus was close to zero, and the removal efficiency of phosphorus reached to above 95%. The operation was stable at this level for 40 d. The results from the SEM indicated that the microbial morphology in the biofilm was uniform with full oval-shaped spheres with a clear profile. MiSeq high-throughput sequencing indicated that the dominant phylum in the reactor included Proteobacteria, Chloroflexi, Bacteroidetes, Actinobacteria, Ignavibacteriae, and Nitrospirae. Proteobacteria, as the dominant genera, increased from 47% to 58%. Rhodocyclaceae, as the dominant phosphorus accumulating bacteria, increased from 17.9% to 28.9%. During the recovery period, the concentration of the phosphorus solution increased from 40mg·L to 82 mg·L by increasing the influent phosphate concentration and the COD concentration in the anaerobic phase, meeting the requirement of phosphorus recovery with the struvite method.
本研究的目的是开发一种从废水中去除和回收高浓度磷酸盐溶液的方法。采用人工布水，以尼龙为生物载体，在生物膜中培养和富集聚磷菌（PAOs）进行实验。通过扫描电子显微镜（SEM）和MiSeq高通量测序研究了生物膜驯化过程中微生物群落形态、物种多样性及生物膜的遗传关系。此外，验证了在短时间内回收传统生物膜中高浓度磷酸盐的可行性。反应器运行10 d时挂膜。挂膜成功后，出水COD低于50 mg·L，出水磷接近零，磷去除率达到95%以上。在此水平下稳定运行40 d。SEM结果表明，生物膜中微生物形态均匀，呈完整的椭圆形球体，轮廓清晰。MiSeq高通量测序表明，反应器中的优势菌门包括变形菌门、绿弯菌门、拟杆菌门、放线菌门、Ignavibacteriae和硝化螺旋菌门。作为优势菌属的变形菌门从47%增加到58%。作为优势聚磷菌的红环菌科从17.9%增加到28.9%。在回收阶段，通过提高厌氧阶段进水磷酸盐浓度和COD浓度，磷溶液浓度从40mg·L增加到82 mg·L，满足了用鸟粪石法回收磷的要求。
