Lu Roujian, Zou Lirong, Wang Yanqun, Zhao Yanie, Zhou Weimin, Wu Jie, Wang Wenling, Wu Guizhen, Ke Changwen, Tan Wenjie
Bing Du Xue Bao. 2015 Jul;31(4):333-40.
To investigate the genetic character and origin of the first imported infection case of middle East respiratory syndrome coronavirus (named as MERS-CoV_China GD01), RNA was extracted from swabs of this patient followed by RT-PCR amplification. All coding gene of structural (S, E, M, E) and accessory (ORF3, ORF4a, ORF4b, ORF5, ORF8b) proteins were sequenced and analyzed. Phylogenetic analyses of structural protein coding genes of MERS-CoV_ China GD01 indicates that several substitutes exists in S coding gene and its origin belong group 5 of MERS-CoV, which were recent circulated in Saudi Arabia area, while other three structural genes (N, E, M) were very conserved. Phylogenetic analyses of accessory protein coding genes of MERS-CoV China GD01 indicates that several substitutes exists among ORF3, ORF4a, ORF4b and ORF5, while ORF8b was conserved. In conclusion, genome of MERS-CoV_ China GD01 was general conserved although several genetic variations were found among structural and accessory protein coding genes. This is the first report on sequencing and phylogenetic analyses of the first imported MERS case in China, which may pay the way for prevention and control of imported MERS-CoV infection.
为研究中东呼吸综合征冠状病毒首例输入性感染病例（命名为MERS-CoV_China GD01）的基因特征及起源，从该患者的拭子中提取RNA，随后进行逆转录聚合酶链反应（RT-PCR）扩增。对结构蛋白（S、E、M、N）和辅助蛋白（ORF3、ORF4a、ORF4b、ORF5、ORF8b）的所有编码基因进行测序和分析。MERS-CoV_China GD01结构蛋白编码基因的系统发育分析表明，S编码基因存在多个替代位点，其起源属于MERS-CoV的第5组，近期在沙特阿拉伯地区流行，而其他三个结构基因（N、E、M）非常保守。MERS-CoV China GD01辅助蛋白编码基因的系统发育分析表明，ORF3、ORF4a、ORF4b和ORF5之间存在多个替代位点，而ORF8b是保守的。总之，尽管在结构和辅助蛋白编码基因中发现了一些基因变异，但MERS-CoV_China GD01的基因组总体上是保守的。这是中国首例输入性中东呼吸综合征病例测序及系统发育分析的首次报道，可为输入性MERS-CoV感染的防控提供思路。
