Kerr Emily, Doeven Egan H, Wilson David J D, Hogan Conor F, Francis Paul S
Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences, Faculty of Science, Engineering and Built Environment, Deakin University, Geelong, Victoria 3220, Australia.
Analyst. 2016 Jan 7;141(1):62-9. doi: 10.1039/c5an01462j. Epub 2015 Nov 3.
The introduction of a 'co-reactant' was a critical step in the evolution of electrogenerated chemiluminescence (ECL) from a laboratory curiosity to a widely utilised detection system. In conjunction with a suitable electrochemiluminophore, the co-reactant enables generation of both the oxidised and reduced precursors to the emitting species at a single electrode potential, under the aqueous conditions required for most analytical applications. The most commonly used co-reactant is tri-n-propylamine (TPrA), which was developed for the classic tris(2,2'-bipyridine)ruthenium(II) ECL reagent. New electrochemiluminophores such as cyclometalated iridium(III) complexes are also evaluated with this co-reactant. However, attaining the excited states in these systems can require much greater energy than that of tris(2,2'-bipyridine)ruthenium(II), which has implications for the co-reactant reaction pathways. In this tutorial review, we describe a simple graphical approach to characterise the energetically feasible ECL pathways with TPrA, as a useful tool for the development of new ECL detection systems.
“共反应剂”的引入是电化学发光（ECL）从实验室新奇事物发展成为广泛应用的检测系统的关键一步。与合适的电化学发光体相结合，共反应剂能够在大多数分析应用所需的水性条件下，于单一电极电位下生成发光物种的氧化态和还原态前体。最常用的共反应剂是三正丙胺（TPrA），它是为经典的三（2,2'-联吡啶）钌（II）ECL试剂而开发的。新型电化学发光体，如环金属化铱（III）配合物，也用这种共反应剂进行评估。然而，在这些体系中达到激发态可能需要比三（2,2'-联吡啶）钌（II）所需能量大得多的能量，这对共反应剂的反应途径有影响。在本教程综述中，我们描述了一种简单的图形方法来表征与TPrA相关的能量上可行的ECL途径，作为开发新型ECL检测系统的有用工具。
