Lazarte Sandra Stella, Mónaco María Eugenia, Jimenez Cecilia Laura, Ledesma Achem Miryam Emilse, Terán Magdalena María, Issé Blanca Alicia
Instituto de Bioquímica Aplicada, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán (UNT), Balcarce 747, San Miguel de Tucumán, 4000 Tucumán, Argentina.
Instituto de Bioquímica Aplicada, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán (UNT), Balcarce 747, San Miguel de Tucumán, 4000 Tucumán, Argentina ; Instituto de Biología, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Chacabuco 461, San Miguel de Tucumán, 4000 Tucumán, Argentina.
Adv Hematol. 2015;2015:343571. doi: 10.1155/2015/343571. Epub 2015 Oct 7.
Most common microcytic hypochromic anemias are iron deficiency anemia (IDA) and β-thalassemia trait (BTT), in which oxidative stress (OxS) has an essential role. Catalase causes detoxification of H2O2 in cells, and it is an indispensable antioxidant enzyme. The study was designed to measure erythrocyte catalase activity (ECAT) in patients with IDA (10) or BTT (21), to relate it with thalassemia mutation type (β (0) or β (+)) and to compare it with normal subjects (67). Ninety-eight individuals were analyzed since September 2013 to June 2014 in Tucumán, Argentina. Total blood count, hemoglobin electrophoresis at alkaline pH, HbA2, catalase, and iron status were performed. β-thalassemic mutations were determined by real-time PCR. Normal range for ECAT was 70,0-130,0 MU/L. ECAT was increased in 14% (3/21) of BTT subjects and decreased in 40% (4/10) of those with IDA. No significant difference (p = 0,245) was shown between normal and BTT groups, while between IDA and normal groups the difference was proved to be significant (p = 0,000). In β (0) and β (+) groups, no significant difference (p = 0,359) was observed. An altered ECAT was detected in IDA and BTT. These results will help to clarify how the catalase activity works in these anemia types.
最常见的小细胞低色素性贫血是缺铁性贫血（IDA）和β地中海贫血特征（BTT），其中氧化应激（OxS）起着至关重要的作用。过氧化氢酶可使细胞中的过氧化氢解毒，它是一种不可或缺的抗氧化酶。本研究旨在测量IDA患者（10例）或BTT患者（21例）的红细胞过氧化氢酶活性（ECAT），将其与地中海贫血突变类型（β（0）或β（+））相关联，并与正常受试者（67例）进行比较。2013年9月至2014年6月在阿根廷图库曼对98名个体进行了分析。进行了全血细胞计数、碱性pH值下的血红蛋白电泳、HbA2、过氧化氢酶和铁状态检测。通过实时PCR确定β地中海贫血突变。ECAT的正常范围为70.0 - 130.0 MU/L。BTT受试者中有14%（3/21）的ECAT升高，IDA患者中有40%（4/10）的ECAT降低。正常组和BTT组之间无显著差异（p = 0.245），而IDA组和正常组之间差异显著（p = 0.000）。在β（0）和β（+）组中，未观察到显著差异（p = 0.359）。在IDA和BTT中检测到ECAT改变。这些结果将有助于阐明过氧化氢酶活性在这些贫血类型中的作用机制。
