Javed Sana, Shahzadi Zainab, Yousaf Zubaida, Anjum Irfan, Aftab Arusa, Hanif Samina, Maqbool Zainab, Ullah Riaz, Raza Muhammad Ahmer, Iqbal Zafar
Department of Botany Lahore College for Women University Lahore Pakistan.
Department of Basic Medical Sciences Shifa College of Pharmaceutical Sciences, Shifa Tameer-e-Millat University Islamabad Pakistan.
Food Sci Nutr. 2024 Jul 21;12(10):7331-7346. doi: 10.1002/fsn3.4314. eCollection 2024 Oct.
Iron deficiency anemia is a global health concern, affecting around 2 billion people. Oral iron therapy often causes severe gastro-intestinal issues. , member of the Brassicaceae family, is valued in traditional medicine and renowned for its rich iron and vitamin C content. This study aims to evaluate the anti-anemic properties of extract in vivo and identify its compounds targeting anemia mechanisms using network pharmacology. Thirty-two Sprague-Dawley rats (200 ± 250 g) were split into two distinct groups, iron-deficient and iron-sufficient. Three different doses (200, 400, and 800 mg/kg) of aqueous extract of were checked against anemia by studying hematological, oxidative stress, and histopathological parameters. GC-MS analysis of revealed its phytochemical profile, followed by ADME screening. Network pharmacology explored targets related to iron deficiency anemia, with oral bioavailability and drug likeness assessment for compounds. The administration of extracts significantly improved various blood parameters, including osmotic fragility, Hb, RBCs, MCV, PCV, and alkaline phosphatase; catalase activity; and histopathological parameters such as liver in both iron-deficient and iron-sufficient rats ( < .001). Seventy-nine compounds were identified in aqueous extract, with only six of them found to be bioavailable and drug-like against multiple targets. Gene ontology and pathway analysis revealed their diverse molecular, biological, and cellular functions. One gene EGFR was found to have functional association with ID anemia, suggesting potential for using extracts. The study concludes that extract has potential for iron deficiency anemia treatment, offering hope for future pharmaceutical interventions.
缺铁性贫血是一个全球性的健康问题，影响着约20亿人。口服铁剂治疗常常会引发严重的胃肠道问题。十字花科植物[此处原文未提及具体植物名称]在传统医学中受到重视，因其富含铁和维生素C而闻名。本研究旨在评估[此处原文未提及具体植物名称]提取物在体内的抗贫血特性，并使用网络药理学确定其针对贫血机制的化合物。32只Sprague-Dawley大鼠（体重200±250克）被分为两组，即缺铁组和铁充足组。通过研究血液学、氧化应激和组织病理学参数，检测了三种不同剂量（200、400和800毫克/千克）的[此处原文未提及具体植物名称]水提取物对贫血的作用。对[此处原文未提及具体植物名称]的气相色谱-质谱分析揭示了其植物化学特征，随后进行了药物代谢动力学筛选。网络药理学探索了与缺铁性贫血相关的靶点，并对化合物进行了口服生物利用度和类药性评估。提取物的给药显著改善了各种血液参数，包括渗透脆性、血红蛋白、红细胞、平均红细胞体积、红细胞压积和碱性磷酸酶；过氧化氢酶活性；以及缺铁和铁充足大鼠肝脏等组织病理学参数（P<0.001）。在[此处原文未提及具体植物名称]水提取物中鉴定出了七十九种化合物，其中只有六种被发现对多个靶点具有生物利用度和类药性。基因本体论和通路分析揭示了它们多样的分子、生物学和细胞功能。发现一个基因表皮生长因子受体（EGFR）与缺铁性贫血存在功能关联，表明[此处原文未提及具体植物名称]提取物具有应用潜力。该研究得出结论，[此处原文未提及具体植物名称]提取物具有治疗缺铁性贫血的潜力，为未来的药物干预提供了希望。
