西咪替丁对牙周炎大鼠模型中硝基氧化应激的影响。
Effect of Cimetidine on Nitro-Oxidative Stress in a Rat Model of Periodontitis.
作者信息
Culic Carina, Parvu Alina Elena, Alb Sandu Florin, Alb Camelia, Pop Angela
机构信息
Department of Odontology, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Department of Pathophysiology, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.
出版信息
Clujul Med. 2014;87(3):177-81. doi: 10.15386/cjmed-273. Epub 2014 Aug 5.
BACKGROUND AND AIMS
Periodontitis is a chronic inflammation that involves nitro-oxidative stress with damaging periodontal structural effects. We aimed to evaluate the consequences of low-dose cimetidine on nitro-oxidative stress in periodontitis.
METHODS
A rat model of ligature-induced periodontitis was used. After two weeks, the periodontitis groups were treated with cimetidine, aminoguanidine, N-nitro-L-arginine methyl ester and trolox for one week. On day 21, blood was drawn and the serum analyzed for measurement of total nitrites and nitrates, total oxidative status, total antioxidant response, and oxidative stress index.
RESULTS
Cimetidine had an inhibitory effect on the synthesis of nitric oxide (p=0.001), total oxidative status (p=0.01) and oxidative stress index (p=0.01). Total antioxidant reactivity was increased by cimetidine (p=0.01). The effects of cimetidine were almost like those of aminoguanidine, NG-nitro-L-arginine methyl ester, and trolox.
CONCLUSIONS
Low-dose cimetidine can be used as adjunctive host modulatory therapy in chronic periodontitis because it reduces nitro-oxidative stress.
背景与目的
牙周炎是一种慢性炎症，涉及氮氧化应激，对牙周结构产生破坏作用。我们旨在评估低剂量西咪替丁对牙周炎中氮氧化应激的影响。
方法
采用结扎诱导的大鼠牙周炎模型。两周后，牙周炎组分别用西咪替丁、氨基胍、N-硝基-L-精氨酸甲酯和曲洛昔康治疗一周。在第21天，采集血液并分析血清，以测量总亚硝酸盐和硝酸盐、总氧化状态、总抗氧化反应和氧化应激指数。
结果
西咪替丁对一氧化氮的合成（p=0.001）、总氧化状态（p=0.01）和氧化应激指数（p=0.01）有抑制作用。西咪替丁可提高总抗氧化反应性（p=0.01）。西咪替丁的作用与氨基胍、N-硝基-L-精氨酸甲酯和曲洛昔康的作用相似。
结论
低剂量西咪替丁可作为慢性牙周炎的辅助宿主调节治疗药物，因为它可降低氮氧化应激。
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