Department of Therapeutic, Orthopedic and Pediatric Dentistry, Zaporizhzhia State Medical University, 69035, Mayakovsky Avenue, 26, Zaporizhzhia, Ukraine.
Department of Pharmacology and Medical Formulation with the Course of Normal Physiology, Zaporizhzhia State Medical University, 69035, Mayakovsky Avenue, 26, Zaporizhzhia, Ukraine.
Microrna. 2024;13(2):132-139. doi: 10.2174/0122115366264794240327073739.
Periodontal diseases are a rather complex problem of modern dentistry and do not have only medical but also social significance. The objective of this study is to weigh the effect of a mixture of Thiotriazoline and L-arginine (1:4) on the parameters of the system of endogenous cytoprotection of blood and periodontal illness in rats with experimental chronic generalized periodontitis and substantiate further study of this blend.
The study aimed to evaluate the impact of a combination of Thiotriazoline and L-arginine (in a ratio of 1:4) on the parameters of the endogenous blood cytoprotection system and periodontium in rats with experimental chronic generalized periodontitis. A group of outbred rats weighing 190-220 g and sourced from the vivarium of the Institute of Pharmacology and Toxicology of the Academy of Medical Sciences of Ukraine were divided into four groups, each consisting of 10 animals. (1) Intact group, animals that were injected intragastrically with a solution of sodium chloride to chloride 0.9% for 30 days. (2) control, animals with experimental CGP who intragastrically sodium chloride solution 0.9% for 30 days. (3) animals with experimental CGP were injected intramuscularly with Thiotriazoline + L-arginine (1:4) in a dosage of 200 mg/kg (30 days). (4) animals with experimental CGP, for which daily intragastric reference drug Mexidol, in dosage 250 mg/kg (30 days). In this study, we utilized two substances: Thiotriazoline and L-arginine hydrochloride. The combination of Thiotriazoline and L-arginine (in a ratio of 1:4) was prepared at the Department of Pharmaceutical Chemistry of ZSMU. At the conclusion of the experiment, the rats were carefully removed from the study while under thiopental-sodium anesthesia, and administered at a dosage of 40 mg/kg.
We have found that the administration of a combined preparation of Thiotriazoline with L-arginine to rats with CGP leads to a significant decrease in the blood concentration of pro-inflammatory cytokines IL-1b and TNF-a by 56.1% and 71%, respectively.
The administration of Mexidol at a dosage of 250 mg/kg, as well as the combination of Thiotriazoline and Larginine in a ratio of 1:4 at a dosage of 200 mg/kg, resulted in a significant reduction in gingival pocket depth in animals with CGP. Specifically, the gingival pocket depth was reduced to 6 mm (p < 0.05) with Mexidol and further reduced to 4 mm (p < 0.05) with the combination of Thiotriazoline and L-arginine. Additionally, the animals exhibited minimal bleeding, swelling, and tooth mobility when treated with the combination of Thiotriazoline and L-arginine. The administration of a combination of Thiotriazoline and L-arginine (in a ratio of 1:4) at a dosage of 200 mg/kg to animals with CGP resulted in a noteworthy reduction in the blood concentration of pro-inflammatory cytokines IL-1b and TNF-a. Specifically, there was a significant decrease of 56.1% (p < 0.05) in IL-1b and 71% (p < 0.05) in TNF-a levels. The course administration of a combination of Thiotriazoline and L-arginine (1:4) (200 mg/kg) to animals with CGP led to an increased expression of HSP70 mRNA (p < 0.05) in the periodontium by 8.2 times and HIF-1a mRNA by 8.2 times. 2.8 times (p < 0.05) against the background of an increase in the blood concentration of HSP70 by 95% (p < 0.05). Also, in the periodontium of animals in this group, a decrease in the expression of c-Fos mRNA by 36.7% (p < 0.05) was found compared to the control group.
牙周病是现代牙科中一个相当复杂的问题，不仅具有医学意义，而且具有社会意义。本研究的目的是衡量混合物硫代三嗪和 L-精氨酸（1:4）对实验性慢性广泛性牙周炎大鼠内源性细胞保护系统参数的影响，并进一步研究这种混合物。
该研究旨在评估硫代三嗪和 L-精氨酸（1:4）混合物对实验性慢性广泛性牙周炎大鼠内源性血液细胞保护系统和牙周参数的影响。一组体重为 190-220 克的杂交大鼠，来自乌克兰医学科学院药理学和毒理学研究所的动物房，分为四组，每组 10 只动物。（1）完整组，动物每天经口给予生理盐水至氯 0.9%，共 30 天。（2）对照组，动物每天经口给予生理盐水 0.9%，共 30 天。（3）实验组 CGP 动物肌肉注射硫代三嗪+L-精氨酸（1:4），剂量为 200mg/kg（30 天）。（4）实验组 CGP 动物，每日经口给予参考药物美西多，剂量为 250mg/kg（30 天）。本研究采用两种物质：硫代三嗪和 L-精氨酸盐酸盐。硫代三嗪和 L-精氨酸（1:4）的混合物在 ZSMU 药物化学系制备。实验结束时，大鼠在硫喷妥钠麻醉下仔细取出，给予 40mg/kg。
我们发现，给予 CGP 大鼠联合制剂硫代三嗪和 L-精氨酸可使促炎细胞因子 IL-1b 和 TNF-a 的血液浓度分别降低 56.1%和 71%。
美西多 250mg/kg 给药以及硫代三嗪与 L-精氨酸以 1:4 的比例联合给药 200mg/kg，可显著降低 CGP 动物的牙龈袋深度。具体来说，用美西多治疗可将牙龈袋深度降低至 6mm（p<0.05），用硫代三嗪和 L-精氨酸联合治疗可进一步降低至 4mm（p<0.05）。此外，用硫代三嗪和 L-精氨酸联合治疗的动物牙龈仅有轻微出血、肿胀和牙齿松动。给予 CGP 动物硫代三嗪和 L-精氨酸（1:4）混合物（200mg/kg）可显著降低促炎细胞因子 IL-1b 和 TNF-a 的血液浓度。具体来说，IL-1b 降低 56.1%（p<0.05），TNF-a 降低 71%（p<0.05）。给予 CGP 动物硫代三嗪和 L-精氨酸（1:4）混合物（200mg/kg）可增加牙周组织中 HSP70 mRNA 的表达（p<0.05），增加 8.2 倍，HIF-1a mRNA 增加 8.2 倍（p<0.05）。在 HSP70 血液浓度增加 95%的基础上（p<0.05），增加 2.8 倍。此外，与对照组相比，该组动物牙周组织中 c-Fos mRNA 的表达降低了 36.7%（p<0.05）。
