超滤法——检测巨泌乳素的一种替代聚乙二醇沉淀法的方法。

Ultrafiltration - an alternative method to polyethylene glycol precipitation for macroprolactin detection.

作者信息

Beda-Maluga Karolina, Pisarek Hanna, Romanowska Irena, Komorowski Jan, Świętosławski Jacek, Winczyk Katarzyna

机构信息

Department of Neuroendocrinology, Interdepartmental Chair of Laboratory and Molecular Diagnostics, Medical University of Lodz, Lodz, Poland.

Institute of Technical Biochemistry, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Lodz, Poland.

出版信息

Arch Med Sci. 2015 Oct 12;11(5):1001-7. doi: 10.5114/aoms.2015.54854.

DOI:10.5114/aoms.2015.54854

PMID:26528343

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4624744/

Abstract

INTRODUCTION

The aim of the study was to evaluate two methods of macroprolactin (MaPRL) detection - precipitation with polyethylene glycol (PEG) and ultrafiltration and to compare these techniques with "gold standard" - gel filtration chromatography (GFC).

MATERIAL AND METHODS

The study was conducted on 245 patients - 45 with organic and 200 with functional hyperprolactinaemia. In all the subjects MaPRL was detected by precipitation with PEG and ultrafiltration. Additionally, gel filtration chromatography was performed in some of the serum samples.

RESULTS

Macroprolactinaemia was detected in 27 patients - 8 with prolactinoma and 19 with functional hyperprolactinaemia. Assessing positive and negative results for MaPRL, we observed high diagnostic agreement (95.9%) and positive correlation (r = 0.506, p < 0.001) between the methods. The results of precipitation and ultrafiltration positive for MaPRL were concordant in 63%. The dominance of MaPRL detected with precipitation and/or ultrafiltration was confirmed by GFC in 76% of cases (all patients with functional hyperprolactinaemia). Among 6 examined patients with prolactinoma, GFC showed four false-positive results - 1 case of precipitation and 3 cases of ultrafiltration.

CONCLUSIONS

Efficacy of MaPRL detection with precipitation and ultrafiltration is comparable especially in cases of functional hyperprolactinaemia. In patients with prolactinoma, precipitation seems to be a more efficient separation method.

摘要

引言

本研究的目的是评估两种检测大分子催乳素（MaPRL）的方法——聚乙二醇（PEG）沉淀法和超滤法，并将这些技术与“金标准”——凝胶过滤色谱法（GFC）进行比较。

材料与方法

对245例患者进行了研究，其中45例患有器质性高催乳素血症，200例患有功能性高催乳素血症。在所有受试者中，通过PEG沉淀法和超滤法检测MaPRL。此外，对部分血清样本进行了凝胶过滤色谱分析。

结果

在27例患者中检测到大分子催乳素血症，其中8例患有催乳素瘤，19例患有功能性高催乳素血症。评估MaPRL的阳性和阴性结果时，我们观察到两种方法之间具有高度的诊断一致性（95.9%）和正相关性（r = 0.506，p < 0.001）。MaPRL沉淀法和超滤法的阳性结果在63%的情况下是一致的。凝胶过滤色谱法在76%的病例（所有功能性高催乳素血症患者）中证实了通过沉淀法和/或超滤法检测到的MaPRL占优势。在6例接受检查的催乳素瘤患者中，凝胶过滤色谱法显示出4例假阳性结果——沉淀法1例，超滤法3例。