Hernández-Bello Jorge, Palafox-Sanchez Claudia A, García-Arellano Samuel, Reyes-Castillo Zyanya, Pereira-Suárez Ana L, Parra-Rojas Isela, Navarro-Zarza José E, De la Cruz-Mosso Ulises, Torres-Carrillo Nora M, Muñoz-Valle José Francisco
Instituto de Investigación en Ciencias Biomédicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, México.
Unidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo de los Bravo, Guerrero, México.
Arch Med Sci. 2018 Aug;14(5):1025-1032. doi: 10.5114/aoms.2016.62138. Epub 2016 Sep 2.
Prolactin (PRL) is a 23-kDa protein that can be synthesized and secreted by pituitary and extrapituitary tissues such as immune cells due to its expression being regulated by two independent promoter regions. The promoter which is responsible for extrapituitary expression contains the single nucleotide polymorphism (SNP) -1149 G/T previously associated with autoimmune diseases in various populations. This study evaluates the relationship of PRL -1149 G/T polymorphism with PRL serum levels and clinical characteristics in systemic lupus erythematosus (SLE) patients from western Mexico.
One hundred and sixty-three SLE patients classified according to the 1982 American College of Rheumatology (ACR) SLE classification criteria and 326 unrelated control subjects (CS), both from western Mexico, were included. The PRL -1149 G/T polymorphism was genotyped using the polymerase chain reaction restriction fragment length polymorphism technique, and both PRL serum levels and autoantibodies were measured by enzyme-linked immunosorbent assay (ELISA).
We found an association between the PRL -1149 TT genotype and SLE according to the recessive genetic model (OR = 2.26, 95% CI: 1.01-5.08, = 0.04). The TT genotype was associated with anti-RNP antibodies ( = 0.04) and with higher scores of the Mex-SLEDAI ( = 0.02). Moreover, SLE patients showed elevated PRL serum levels (12.4 ng/ml; < 0.01), and this condition was associated with renal activity and the presence of anti-RNP antibodies.
PRL -1149 TT genotype is associated with susceptibility to SLE in a Mexican-Mestizo population, and high PRL serum levels are associated with anti-RNP antibodies and renal activity.
催乳素（PRL）是一种23 kDa的蛋白质，由于其表达受两个独立启动子区域调控，可由垂体及垂体外组织（如免疫细胞）合成和分泌。负责垂体外表达的启动子包含单核苷酸多态性（SNP）-1149 G/T，先前已在不同人群中发现其与自身免疫性疾病相关。本研究评估墨西哥西部系统性红斑狼疮（SLE）患者中PRL -1149 G/T多态性与PRL血清水平及临床特征的关系。
纳入163例根据1982年美国风湿病学会（ACR）SLE分类标准分类的SLE患者以及326例无关对照受试者（CS），均来自墨西哥西部。采用聚合酶链反应-限制性片段长度多态性技术对PRL -1149 G/T多态性进行基因分型，通过酶联免疫吸附测定（ELISA）检测PRL血清水平和自身抗体。
根据隐性遗传模型，我们发现PRL -1149 TT基因型与SLE之间存在关联（OR = 2.26，95% CI：1.01 - 5.08，P = 0.04）。TT基因型与抗RNP抗体相关（P = 0.04），且与Mex-SLEDAI评分较高相关（P = 0.02）。此外，SLE患者PRL血清水平升高（12.4 ng/ml；P < 0.01），且这种情况与肾脏活动及抗RNP抗体的存在相关。
在墨西哥梅斯蒂索人群中，PRL -1149 TT基因型与SLE易感性相关，高PRL血清水平与抗RNP抗体及肾脏活动相关。
