人中性粒细胞的分离与功能分析
Isolation and Functional Analysis of Human Neutrophils.
作者信息
Kuhns Douglas B, Priel Debra A Long, Chu Jessica, Zarember Kol A
机构信息
Neutrophil Monitoring Lab, Leidos Biomedical Research Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland.
Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.
出版信息
Curr Protoc Immunol. 2015 Nov 2;111:7.23.1-7.23.16. doi: 10.1002/0471142735.im0723s111.
Abstract
This unit describes the isolation of human polymorphonuclear neutrophils (PMN) from blood using dextran sedimentation and Percoll or Ficoll-Paque density gradients. Assays of neutrophil functions including respiratory burst activation, phagocytosis, and microbial killing are also described.
摘要
本单元介绍了使用葡聚糖沉降法以及Percoll或Ficoll-Paque密度梯度从血液中分离人多形核中性粒细胞（PMN）的方法。同时还描述了中性粒细胞功能的检测方法，包括呼吸爆发激活、吞噬作用和微生物杀伤。
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