Dohle Eva, Zuo Hongyu, Bayrak Büşra, Heselich Anja, Schäfer Birgit, Sader Robert, Ghanaati Shahram
FORM, Frankfurt Orofacial Regenerative Medicine, Department for Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Johann Wolfgang Goethe University, 60590 Frankfurt, Germany.
ABIS e.V., Academy for Biological Innovations in Surgery Formally Known as SBCB e.V., Society for Blood Concentrates and Biomaterials e.V., 60435 Frankfurt, Germany.
Biomedicines. 2025 May 20;13(5):1239. doi: 10.3390/biomedicines13051239.
: The body's reaction after the implantation of a biomaterial is a non-specific inflammatory response that is mainly initiated via the recruitment of polymorphonuclear cells (PMNs) to the implant site secreting cytokines and growth factors, followed by activation of monocytes/macrophages, finally leading to wound healing. The wound healing process is dependent on the priming of the PMNs that can be guided towards an inflammatory or a regenerative phenotype with the associated characteristic PMN cytokine profiles. Since the collagen-based Purified Reconstituted Bilayer Matrix (PRBM) triggers the wound healing process at the implant site in vivo, it is hypothesized that this positive effect might be due to a material-mediated priming of the PMNs towards the regenerative phenotype. With the use of the blood concentrate platelet-rich fibrin (PRF) containing high concentrations of leukocytes, including PMNs, the natural environment of the body after the implantation of a material can be mimicked in vitro. The aim of the present study was to characterize the phenotype of native blood-derived PMNs within PRF in response to the PRBM. : PMNs within PRF gained from different relative centrifugal forces were characterized in a first step before PRF was combined with the PRBM for 4 h. Supernatants were harvested to analyze the phenotype of the PMNs via the evaluation of eight different cytokines using the ELISA. : Analysis of the PMN phenotype could assess cytokines commonly associated with neutrophils of the proinflammatory phenotype, such as TNFα, IL15, and IL1, as lower in supernatants when PRF was incubated in the presence of the PRBM and compared to the control PRF. On the other hand, cytokines related to the PMN regenerative phenotype, like TGFβ and IL10, could be detected as higher when PRF was incubated in the presence of the PRBM. : This might suggest that PRBM significantly activates and primes neutrophils to the regenerative phenotype, leading to the resolution of inflammation. This might trigger the process of wound healing and tissue regeneration, making the PRBM a beneficial material for therapeutic applications.
生物材料植入后机体的反应是一种非特异性炎症反应，主要通过多形核细胞（PMN）募集至植入部位并分泌细胞因子和生长因子启动，随后单核细胞/巨噬细胞被激活，最终导致伤口愈合。伤口愈合过程取决于PMN的预激活，PMN可通过相关特征性的PMN细胞因子谱被引导至炎症或再生表型。由于基于胶原蛋白的纯化重组双层基质（PRBM）在体内可触发植入部位的伤口愈合过程，因此推测这种积极作用可能是由于材料介导的PMN向再生表型的预激活。通过使用含有高浓度白细胞（包括PMN）的富血小板纤维蛋白（PRF）血液浓缩物，可在体外模拟材料植入后机体的自然环境。本研究的目的是表征PRF中天然血液来源的PMN对PRBM的反应表型。：在将PRF与PRBM结合4小时之前，第一步是对从不同相对离心力获得的PRF中的PMN进行表征。收集上清液，通过酶联免疫吸附测定（ELISA）评估八种不同的细胞因子来分析PMN的表型。：对PMN表型的分析可以评估通常与促炎表型中性粒细胞相关的细胞因子，如肿瘤坏死因子α（TNFα）、白细胞介素15（IL15）和白细胞介素1（IL1），当PRF在PRBM存在下孵育时，与对照PRF相比，上清液中的这些细胞因子含量较低。另一方面，当PRF在PRBM存在下孵育时，可以检测到与PMN再生表型相关的细胞因子，如转化生长因子β（TGFβ）和白细胞介素10（IL10）含量较高。：这可能表明PRBM显著激活并将中性粒细胞预激活为再生表型，从而导致炎症消退。这可能触发伤口愈合和组织再生过程，使PRBM成为治疗应用中的有益材料。
