Tummalapalli Mythili, Berthet Morgane, Verrier Bernard, Deopura B L, Alam M S, Gupta Bhuvanesh
Bioengineering Lab, Department of Textile Technology, Indian Institute of Technology, New Delhi 16, India.
Laboratory of Tissue Biology and Therapeutic Engineering, IBCP, UMR 5305 CNRS-UCBL, Lyon Cedex 07, France.
Int J Biol Macromol. 2016 Jan;82:104-13. doi: 10.1016/j.ijbiomac.2015.10.087. Epub 2015 Nov 1.
Aloe vera and curcumin loaded oxidized pectin-gelatin (OP-Gel) matrices were used as antimicrobial finishes on nonwoven cotton fabrics to produce composite wound care devices. The drug release characteristics of the biocomposite dressings indicated that curcumin is released through a biphasic mechanism - erosion of the polymeric matrix, followed by diffusion, while aloe vera is released upon leaching of the polymeric matrix. A 50/50 composition of aloe vera/curcumin was used to fabricate OP-Gel-Aloe Curcumin dressings. However, contrary to our expectations, OP-Gel-Aloe Curcumin dressings exhibited lesser antimicrobial activity compared to OP-Gel-Aloe and OP-Gel-Curcumin dressings. The cytocompatibility of the fabricated dressings was evaluated using NIH3T3 mouse fibroblast cells. OP-Gel-Aloe treated fibroblasts had the highest viability, with the matrices providing a substrate for good cell attachment and proliferation. On the other hand, OP-Gel-Curcumin and OP-Gel-Aloe Curcumin seemed to have induced apoptosis in NIH3T3 cells. In vivo wound healing analysis was carried out using an excisional splint wound model on C57BL/6J mice. OP-Gel-Aloe treated wounds exhibited very rapid healing with 80% of the wound healing in just 8 days. Furthermore, aloe vera exerted a strong anti-inflammatory effect and prominent scar prevention. Histological examination revealed that an ordered collagen formation and neovascularization could be observed along with migration of nuclei. Therefore, OP-Gel-Aloe biocomposite dressings are proposed as viable materials for effective wound management.
负载芦荟和姜黄素的氧化果胶-明胶（OP-Gel）基质被用作非织造棉织物的抗菌整理剂，以生产复合伤口护理装置。生物复合敷料的药物释放特性表明，姜黄素通过双相机制释放——聚合物基质侵蚀，随后扩散，而芦荟在聚合物基质浸出时释放。采用50/50的芦荟/姜黄素组合物制备OP-Gel-芦荟姜黄素敷料。然而，与我们的预期相反，与OP-Gel-芦荟敷料和OP-Gel-姜黄素敷料相比，OP-Gel-芦荟姜黄素敷料的抗菌活性较低。使用NIH3T3小鼠成纤维细胞评估所制备敷料的细胞相容性。OP-Gel-芦荟处理的成纤维细胞具有最高的活力，这些基质为细胞良好的附着和增殖提供了底物。另一方面，OP-Gel-姜黄素和OP-Gel-芦荟姜黄素似乎诱导了NIH3T3细胞的凋亡。使用切除夹板伤口模型在C57BL/6J小鼠上进行体内伤口愈合分析。OP-Gel-芦荟处理的伤口愈合非常迅速，仅8天就有80%的伤口愈合。此外，芦荟具有很强的抗炎作用和显著的防疤效果。组织学检查显示，可以观察到有序的胶原形成和新血管形成以及细胞核迁移。因此，OP-Gel-芦荟生物复合敷料被认为是有效伤口管理的可行材料。
