Liu Feng, Deng Ming, Li Jiale, Zhang Ziying, Deng Yefeng, Lv Lianpeng, Li Yichang, Du Hao, Zhang Chao, Li Junting, Wang Bing
Department of Polymer Materials, Zhejiang Sci-Tech University, Hangzhou 310018, China; Zhejiang Sci-Tech University Shengzhou Innovation Research Institute, Shengzhou 312451, China.
Department of Polymer Materials, Zhejiang Sci-Tech University, Hangzhou 310018, China.
Int J Biol Macromol. 2024 Aug;275(Pt 2):133251. doi: 10.1016/j.ijbiomac.2024.133251. Epub 2024 Jun 30.
Bioactive hydrogels are currently receiving significant attention. In this study, silk fibroin tyramine-modified gelatin hydrogels (SF-TG) with varying degrees of tyramine root substitution were explored. The physicochemical property and biocompatibility of low degree of substitution tyramine-modified gelatin hydrogel (SF-LTG) and high degree of substitution tyramine-modified gelatin hydrogel (SF-HTG) were compared. The results showed that SF-LTG possessed better mechanical property and higher biocompatibility. Thus, SF-LTG was selected as a bioactive matrix and loaded with basic fibroblast growth factor (bFGF); subsequently, curcumin-coupled chitosan rods (CCCRs-EGF) enriched with epidermal growth factor (EGF) were added to obtain SF-LTG-bFGF@CCCRs-EGF hydrogels. The results showed that SF-LTG-bFGF@CCCRs-EGF retained the basic structural and mechanical properties of the SF-LTG matrix gel material and underwent multiple loading and orderly release with different activities while displaying antioxidant, anti-inflammatory, antimicrobial, and pro-cellular proliferation activities and orderly regulation of activity during wound healing. Therefore, the SF-LTG-bFGF@CCCRs-EGF hydrogel is of great value in healing complex wounds.
生物活性水凝胶目前正受到广泛关注。在本研究中，探索了具有不同程度酪胺根取代的丝素蛋白酪胺修饰明胶水凝胶（SF-TG）。比较了低取代度酪胺修饰明胶水凝胶（SF-LTG）和高取代度酪胺修饰明胶水凝胶（SF-HTG）的物理化学性质和生物相容性。结果表明，SF-LTG具有更好的机械性能和更高的生物相容性。因此，选择SF-LTG作为生物活性基质并负载碱性成纤维细胞生长因子（bFGF）；随后，添加富含表皮生长因子（EGF）的姜黄素偶联壳聚糖棒（CCCRs-EGF）以获得SF-LTG-bFGF@CCCRs-EGF水凝胶。结果表明，SF-LTG-bFGF@CCCRs-EGF保留了SF-LTG基质凝胶材料的基本结构和机械性能，并在具有不同活性的情况下进行多次负载和有序释放，同时在伤口愈合过程中表现出抗氧化、抗炎、抗菌和促进细胞增殖活性以及对活性的有序调节。因此，SF-LTG-bFGF@CCCRs-EGF水凝胶在治疗复杂伤口方面具有重要价值。
