乙型肝炎患者中微小RNA-196A2和微小RNA-146A多态性与肝硬化及肝细胞癌进展之间的关联
Association between microRNA-196A2 and microRNA-146A polymorphisms and progression to cirrhosis and hepatocellular carcinoma in patients with viral hepatitis B.
作者信息
Riazalhosseini Behnaz, Mohamed Zahurin, Apalasamy Yamunah D, Eng Hooi Sian, Mohamed Rosmawati
机构信息
aThe Pharmacogenomics Laboratory, Department of Pharmacology bDepartment of Medicine cDepartment of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
出版信息
Pharmacogenet Genomics. 2016 Feb;26(2):74-9. doi: 10.1097/FPC.0000000000000187.
BACKGROUND/AIM: MicroRNAs (miRNAs) are small noncoding RNAs that have been implicated in mechanisms underlying various types of cancers including hepatocellular carcinoma (HCC). Reports have indicated that single nucleotide polymorphisms in miRNA-196A2 and miRNA-146A genes may contribute to the risk of progression of hepatitis B virus (HBV) infection to cirrhosis and HCC. This study aimed to examine the effect of miRNA-196A2 and miRNA-146A polymorphisms on the progression of HBV infection to cirrhosis and/or HCC in HBV patients in the Malaysian population.
PATIENTS AND METHODS
This study consists of 423 chronic HBV patients without either cirrhosis or HCC and 103 chronic HBV patients diagnosed with liver cirrhosis or with cirrhosis and HCC. The single nucleotide polymorphisms of miRNA-196A2 (rs12304647 and rs11614913) and miRNA-146A (rs2910164) were genotyped using the Sequenom MassARRAY platform.
RESULTS
The genotype distribution in chronic HBV without either cirrhosis or HCC, relative to chronic HBV patients diagnosed with liver cirrhosis or with cirrhosis and HCC revealed that rs12304647 has a protective effect from the development of HCC (odds ratio=0.37, 95% confidence interval=0.15-0.89, P=0.027). However, rs11614913 and rs2910164 were not significantly associated with progression of the HBV infection.
CONCLUSION
In summary, rs12304647 is associated with a reduced risk of progression to HCC in patients with chronic HBV infection.
背景/目的：微小RNA（miRNA）是一类小的非编码RNA，与包括肝细胞癌（HCC）在内的多种癌症的发病机制有关。报告表明，miRNA - 196A2和miRNA - 146A基因中的单核苷酸多态性可能会增加乙型肝炎病毒（HBV）感染发展为肝硬化和HCC的风险。本研究旨在探讨miRNA - 196A2和miRNA - 146A多态性对马来西亚人群中HBV患者HBV感染发展为肝硬化和/或HCC的影响。
患者和方法
本研究包括423例无肝硬化或HCC的慢性HBV患者以及103例被诊断为肝硬化或肝硬化合并HCC的慢性HBV患者。使用Sequenom MassARRAY平台对miRNA - 196A2（rs12304647和rs11614913）和miRNA - 146A（rs2910164）的单核苷酸多态性进行基因分型。
结果
与被诊断为肝硬化或肝硬化合并HCC的慢性HBV患者相比，无肝硬化或HCC的慢性HBV患者的基因型分布显示，rs12304647对HCC的发生具有保护作用（比值比=0.37，95%置信区间=0.15 - 0.89，P = 0.027）。然而，rs11614913和rs2910164与HBV感染的进展无显著相关性。
结论
总之，rs12304647与慢性HBV感染患者进展为HCC的风险降低有关。
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