Schwarz S, Spies U, Reitz B, Seyfert H M, Lämmler C, Blobel H
Institut für Bakteriologie und Immunologie, Justus-Liebig-Universität Giessen.
Zentralbl Bakteriol Mikrobiol Hyg A. 1989 Mar;270(4):462-9. doi: 10.1016/s0176-6724(89)80017-3.
Pasteurella haemolytica-cultures, isolated from cattle with respiratory diseases, were investigated for their biotype, serotype, antimicrobial resistance and plasmid content. A plasmid encoding a beta-lactamase could be demonstrated in 9 of 19 Pasteurella haemolytica-cultures. These 9 cultures, all belonging to biotype A and serotype 1, were resistant to ampicillin, carbenicillin, penicillin G and ticarcillin. The plasmid of the respective cultures proved to be identical upon Southern blot hybridization. It could be transformed into Escherichia coli 490 A where it expressed again a beta-lactamase-activity.
从患有呼吸道疾病的牛身上分离出溶血巴斯德氏菌培养物，对其生物型、血清型、抗菌药物耐药性和质粒含量进行了研究。在19株溶血巴斯德氏菌培养物中，有9株可证明含有编码β-内酰胺酶的质粒。这9株培养物均属于生物型A和血清型1，对氨苄西林、羧苄西林、青霉素G和替卡西林耐药。经Southern印迹杂交证明，各培养物的质粒是相同的。它可以转化到大肠杆菌490 A中，并在其中再次表达β-内酰胺酶活性。
