外源性人类ras和myc癌基因对大鼠嗜铬细胞瘤PC12细胞形态分化的影响。

The effect of exogenous human ras and myc oncogenes in morphological differentiation of the rat pheochromocytoma PC12 cells.

作者信息

Spandidos D A

机构信息

Beatson Institute for Cancer Research, Bearsden, Glasgow, Scotland, U.K.

出版信息

Int J Dev Neurosci. 1989;7(1):1-4. doi: 10.1016/0736-5748(89)90039-7.

DOI:10.1016/0736-5748(89)90039-7

PMID:2652983

Abstract

An electroporation technique was employed to study the effect of oncogenes H-ras and c-myc after their short-term expression in the rat pheochromocytoma PC12 cells. It was found that within 6 days after electroporation the mutant T24 H-ras 1 gene induced differentiation of PC12 cells whereas the c-myc blocked NGF-induced differentiation. The induction of differentiation by the T24 H-ras gene may suggest a physiological role of the ras gene in cell differentiation as well as in cell proliferation.

摘要

采用电穿孔技术研究癌基因H-ras和c-myc在大鼠嗜铬细胞瘤PC12细胞中短期表达后的作用。结果发现，电穿孔后6天内，突变型T24 H-ras 1基因诱导PC12细胞分化，而c-myc阻断神经生长因子（NGF）诱导的分化。T24 H-ras基因诱导分化可能提示ras基因在细胞分化以及细胞增殖中具有生理作用。