Lin Zachary Yu-Ching, Hikabe Orie, Suzuki Sadafumi, Hirano Takamasa, Siomi Haruhiko, Sasaki Erika, Imamura Masanori, Okano Hideyuki
Department of Physiology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan.
Department of Molecular Biology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan.
Primates. 2016 Jan;57(1):129-35. doi: 10.1007/s10329-015-0500-4. Epub 2015 Nov 3.
Spermatogonia are specialized cells responsible for continuous spermatogenesis and the production of offspring. Because of this biological property, in vitro culture of spermatogonia provides a powerful methodology to advance reproductive biology and engineering. However, methods for culturing primate spermatogonia are poorly established. We have designed a novel method for culturing spermatogonia in the common marmoset (Callithrix jacchus), a small primate. By using our method with a suite of growth factors, adult marmoset testis-derived germ cells could be cultured in the form of a floating sphere for several weeks. Notably, this method could be applied not only to freshly isolated cells but also to cryopreserved cell stocks. The spheres enriched spermatogonia and early spermatocytes, and could be assembled from a C-KIT(+) spermatogonial population. Techniques for culturing spermatogonia could facilitate increased understanding of primate reproduction as well as the preservation of valuable biomaterials from nonhuman primates.
精原细胞是负责持续精子发生和产生后代的特殊细胞。由于这种生物学特性，精原细胞的体外培养为推进生殖生物学和工程学提供了一种强大的方法。然而，灵长类精原细胞的培养方法尚未完善。我们设计了一种在普通狨猴（Callithrix jacchus）这种小型灵长类动物中培养精原细胞的新方法。通过使用我们的方法和一系列生长因子，成年狨猴睾丸来源的生殖细胞可以以悬浮球的形式培养数周。值得注意的是，这种方法不仅可以应用于新鲜分离的细胞，也可以应用于冷冻保存的细胞库。这些球体富集了精原细胞和早期精母细胞，并且可以从C-KIT(+)精原细胞群体组装而成。精原细胞培养技术有助于增进对灵长类生殖的理解，以及保存来自非人类灵长类动物的宝贵生物材料。
