荧光RNA导入活细胞线粒体

Import of Fluorescent RNA into Mitochondria of Living Cells.

作者信息

Zelenka Jaroslav, Ježek Petr

机构信息

Department No. 75, Membrane Transprot Biophysics, Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1084, Prague 4, 14220, Czech Republic.

出版信息

Methods Mol Biol. 2016;1351:175-81. doi: 10.1007/978-1-4939-3040-1_13.

DOI:10.1007/978-1-4939-3040-1_13

PMID:26530682

Abstract

Methods of in vivo visualization and manipulation of mitochondrial genetic machinery are limited due to the need to surpass not only the cytoplasmic membrane but also two mitochondrial membranes. Here, we employ the matrix-addressing sequence of mitochondrial ribosomal 5S-rRNA (termed MAM), which is naturally imported into mammalian mitochondria, to construct an import system for in vivo targeting of mitochondrial (mt) DNA or mtRNA, in order to provide fluorescence hybridization of the desired sequences.

摘要

由于不仅需要跨越细胞质膜，还需跨越两层线粒体膜，用于体内可视化和操纵线粒体遗传机制的方法受到限制。在此，我们利用天然导入哺乳动物线粒体的线粒体核糖体5S - rRNA的基质靶向序列（称为MAM），构建了一个用于体内靶向线粒体（mt）DNA或mtRNA的导入系统，以实现所需序列的荧光杂交。