Li J Q, Wang L H, Zhan Q W, Liu Y L, Zhang Q, Li J F, Fan F F
College of Agriculture, Anhui Science and Technology University, Fengyang, China.
Genet Mol Res. 2015 Oct 27;14(4):13266-73. doi: 10.4238/2015.October.26.23.
The identification of quantitative trait loci (QTLs) affecting forage quality traits enables an understanding of the genetic mechanism of these loci. The aim of the present study was to detect QTLs for the whole-plant protein content (WP), whole-plant fat content (WF), neutral detergent fiber (NDF), acid detergent fiber (ADF), and whole-plant ash content (WA) using a population of 184 F2 individuals from a cross between sorghum Tx623A and sudangrass Sa. Correlation analysis was performed between the five forage quality traits. WP was found to be positively correlated with WF, NDF, and ADF. Furthermore, NDF was positively correlated with ADF but negatively correlated with WA. A genetic map with 124 SSR markers was constructed for QTL mapping. A total of 12 QTLs associated with the five forage quality traits were detected. Of these QTLs, qNDF3, qNDF8, and qADF8 explained more than 10% of the phenotypic variation. Additionally, although all of the QTLs exhibited additive and dominant effects, they mainly exhibited dominant effects. Our results provide important information for marker-assisted selection breeding of sorghum-sudangrass hybrids.
鉴定影响牧草品质性状的数量性状位点（QTL）有助于了解这些位点的遗传机制。本研究的目的是利用高粱Tx623A与苏丹草Sa杂交产生的184个F2个体群体，检测全株蛋白质含量（WP）、全株脂肪含量（WF）、中性洗涤纤维（NDF）、酸性洗涤纤维（ADF）和全株灰分含量（WA）的QTL。对这五个牧草品质性状进行了相关性分析。发现WP与WF、NDF和ADF呈正相关。此外，NDF与ADF呈正相关，但与WA呈负相关。构建了一个包含124个SSR标记的遗传图谱用于QTL定位。共检测到12个与这五个牧草品质性状相关的QTL。在这些QTL中，qNDF3、qNDF8和qADF8解释了超过10%的表型变异。此外，虽然所有QTL都表现出加性和显性效应，但它们主要表现为显性效应。我们的结果为高粱-苏丹草杂交种的标记辅助选择育种提供了重要信息。
