Agricultural College, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
PLoS One. 2022 Nov 29;17(11):e0278153. doi: 10.1371/journal.pone.0278153. eCollection 2022.
The sorghum-sudangrass hybrid is a vital annual gramineous herbage. Few reports exist on its ultra-high-density genetic map. In this study, we sought to create an ultra-high-density genetic linkage map for this hybrid to strengthen its functional genomics research and genetic breeding. We used 150 sorghum-sudangrass hybrid F2 individuals and their parents (scattered ear sorghum and red hull sudangrass) for high-throughput sequencing on the basis of whole genome resequencing. In total, 1,180.66 Gb of data were collected. After identification, filtration for integrity, and partial segregation, over 5,656 single nucleotide polymorphism markers of high quality were detected. An ultra-high-density genetic linkage map was constructed using these data. The markers covered approximately 2,192.84 cM of the map with average marker intervals of 0.39 cM. The length ranged from 115.39 cM to 264.04 cM for the 10 linkage groups. Currently, this represents the first genetic linkage map of this size, number of molecular markers, density, and coverage for sorghum-sudangrass hybrid. The findings of this study provide valuable genome-level information on species evolution and comparative genomics analysis and lay the foundation for further research on quantitative trait loci fine mapping and gene cloning and marker-assisted breeding of important traits in sorghum-sudangrass hybrids.
高粱-苏丹草杂种是一种重要的一年生禾本科牧草。关于其超高密度遗传图谱的报道很少。在这项研究中，我们试图为该杂种创建一个超高密度遗传连锁图谱，以加强其功能基因组学研究和遗传育种。我们基于全基因组重测序，使用 150 个高粱-苏丹草杂种 F2 个体及其亲本（散穗高粱和红壳苏丹草）进行高通量测序。共收集了 1180.66Gb 的数据。经过完整性鉴定、过滤和部分分离，检测到了超过 5656 个高质量的单核苷酸多态性标记。使用这些数据构建了一个超高密度遗传连锁图谱。标记覆盖了大约 2192.84cM 的图谱，平均标记间隔为 0.39cM。10 个连锁群的长度范围从 115.39cM 到 264.04cM。目前，这是高粱-苏丹草杂种首次构建的具有如此大小、分子标记数量、密度和覆盖度的遗传连锁图谱。这项研究的结果为物种进化和比较基因组分析提供了有价值的基因组水平信息，为高粱-苏丹草杂种重要性状的数量性状位点精细定位、基因克隆和分子标记辅助育种的进一步研究奠定了基础。
