Du W X, Qin Y C
Department of Entomology, China Agricultural University, Beijing, China.
Genet Mol Res. 2015 Oct 27;14(4):13375-9. doi: 10.4238/2015.October.26.35.
Nuclear mitochondrial pseudogenes (numts), which originated from mitochondrial DNA (mtDNA) insertions in the nuclear genome, have been detected in many species. The distribution of numts in the honeybee nuclear genome has not yet been fully reported. By referring to the whole honeybee mtDNA sequence and to the recent version of the honeybee nuclear genome, 236 reference sequences were identified by BLAST, with 90 unmapped. The size of the numts ranged from 219 to 3788 bp, and the homologous identity between numts and their corresponding mtDNA fragments varied from 71 to 93%. Furthermore, identified honeybee numts covered nearly all mitochondrial genes and were distributed over all chromosomes. This study provides useful information for further research related to mitochondrial genes and the evolution of the honeybee.
核线粒体假基因（numts）起源于线粒体DNA（mtDNA）插入核基因组，已在许多物种中被检测到。蜜蜂核基因组中numts的分布尚未得到充分报道。通过参考整个蜜蜂mtDNA序列和最新版本的蜜蜂核基因组，利用BLAST鉴定出236个参考序列，其中90个未定位。numts的大小范围为219至3788 bp，numts与其相应的mtDNA片段之间的同源性从71%到93%不等。此外，鉴定出的蜜蜂numts几乎覆盖了所有线粒体基因，并分布在所有染色体上。本研究为进一步研究蜜蜂线粒体基因和进化提供了有用信息。
