Liu Y, Zhao X
State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, China.
J Anim Breed Genet. 2007 Oct;124(5):264-8. doi: 10.1111/j.1439-0388.2007.00674.x.
The nuclear mitochondrial pseudogenes (numts), originated from mtDNA insertions into the nuclear genome, have been detected to exist in many species. However, the distribution of numts in cattle nuclear genome yet has not been fully reported. By referring to the whole cattle mtDNA sequence and to the recently released cattle nuclear genome by Human Genome Sequencing Center (HGSC), 303 numts were identified by BLAST with 55 numts unmapped to cattle nuclear genome. Further analysis found that the size of the numts ranges from 37 to 1932 bp, and the homologous identity between numts and their corresponding mtDNA fragments varies from 73 to 98%. Furthermore, the identified cattle numts cover nearly all the mitochondrial genes including mtDNA control region, distributing over all the chromosomes with the exception of the chromosome 23 and Y chromosome (in the latter the sequence data are not available). In the discovered numts, 29 relatively complete mitochondrial genes, which were distributed in 72 numts, were detected. Undoubtedly, this research would provide some valuable information for successive research related to mitochondrial genes and the evolution of cattle.
核线粒体假基因（numts）起源于线粒体DNA插入核基因组，已在许多物种中被检测到存在。然而，numts在牛核基因组中的分布尚未得到充分报道。通过参考完整的牛线粒体DNA序列以及人类基因组测序中心（HGSC）最近发布的牛核基因组，利用BLAST鉴定出303个numts，其中55个未定位到牛核基因组。进一步分析发现，numts的大小范围为37至1932 bp，numts与其相应线粒体DNA片段之间的同源性在73%至98%之间。此外，鉴定出的牛numts几乎覆盖了所有线粒体基因，包括线粒体DNA控制区，分布在除23号染色体和Y染色体（后者无序列数据）之外的所有染色体上。在所发现的numts中，检测到29个相对完整的线粒体基因，分布在72个numts中。毫无疑问，这项研究将为与线粒体基因和牛的进化相关的后续研究提供一些有价值的信息。
