He Y F, Liu F Y, Zhang W X
The First Department of Pediatrics, Central Hospital of Xinxiang City, Xinxiang, Henan, China.
The First Department of Pediatrics, Central Hospital of Xinxiang City, Xinxiang, Henan, China
Genet Mol Res. 2015 Oct 29;14(4):13642-8. doi: 10.4238/2015.October.28.26.
The treatment of obese patients is a topic investigated by an increasing number of researchers. This study aimed to elucidate the possible inhibitory effect of tangeritin on the development and function of fat cells. 3T3-L1 fat cells were grown to confluence and subjected to different concentrations of tangeritin. The most effective tangeritin inhibition concentration was determined by the MTT assay. The treated cells were subjected to real-time reverse transcriptase PCR and western blot analysis, to detect changes in the CCAAT/enhancer binding protein (C/EBP)α, C/EBPβ, and peroxisome proliferator activated receptor (PPAR)γ expression levels. The MTT assay revealed that the fat cell growth was inhibited at a 20 ng/mL concentration of tangeritin. The results of real-time PCR revealed a significant decrease in the expression of C/EBPα, C/EBPβ, and PPARγ mRNA, following the treatment with tangeritin. Western blot analysis also presented similar results at a protein level. Therefore, we concluded that tangeritin inhibits adipogenesis via the down-regulation of C/EBPα, C/EBPβ, and PPARγ mRNA and protein expression in 3T3-L1 cells.
肥胖患者的治疗是越来越多研究人员所探讨的一个课题。本研究旨在阐明橘皮素对脂肪细胞发育和功能可能的抑制作用。将3T3-L1脂肪细胞培养至汇合状态，并给予不同浓度的橘皮素。通过MTT法确定橘皮素的最有效抑制浓度。对处理后的细胞进行实时逆转录聚合酶链反应和蛋白质印迹分析，以检测CCAAT/增强子结合蛋白（C/EBP）α、C/EBPβ和过氧化物酶体增殖物激活受体（PPAR）γ表达水平的变化。MTT法显示，在20 ng/mL橘皮素浓度下脂肪细胞生长受到抑制。实时聚合酶链反应结果显示，用橘皮素处理后，C/EBPα、C/EBPβ和PPARγ mRNA的表达显著降低。蛋白质印迹分析在蛋白质水平上也呈现出类似结果。因此，我们得出结论，橘皮素通过下调3T3-L1细胞中C/EBPα、C/EBPβ和PPARγ mRNA及蛋白质表达来抑制脂肪生成。
