Wang Y H, Li X D, Zhu W B, Sun G F
Department of Anesthesiology, Binzhou People's Hospital, Binzhou, Shandong, China.
Genet Mol Res. 2015 Oct 30;14(4):13852-9. doi: 10.4238/2015.October.29.5.
The objective of this study was to examine the effect of high-intensity exercise on interleukin-15 (IL-15) expression in rabbit synovia. We utilized 24 New Zealand white rabbits, which were randomly divided equally into high-intensity exercise and control groups. The former were forced to run for 60 min/day over 4 weeks at the speed of 30 m/min. The histological changes of cartilage and knee joint synovia were investigated with hematoxylin and eosin staining. Immunohistochemistry and enzyme-linked immunosorbent assays were performed to measure IL-15 expression. From these analyses, we identified knee articular cartilage damage and synovitis in the high-intensity exercise group. This group also exhibited higher IL-15 expression in their synovial fluid and tissues than was observed in the control group (P < 0.05). These results suggested that high-intensity exercise might lead to synovitis and articular cartilage damage, and that IL-15 overexpression in synovia might be associated with post-traumatic osteoarthritis.
本研究的目的是检测高强度运动对兔滑膜中白细胞介素-15（IL-15）表达的影响。我们选用了24只新西兰白兔，将其随机平均分为高强度运动组和对照组。前者被迫以30米/分钟的速度每天跑步60分钟，持续4周。采用苏木精-伊红染色法研究软骨和膝关节滑膜的组织学变化。进行免疫组织化学和酶联免疫吸附测定以检测IL-15的表达。通过这些分析，我们在高强度运动组中发现了膝关节软骨损伤和滑膜炎。该组滑膜液和组织中的IL-15表达也高于对照组（P <0.05）。这些结果表明，高强度运动可能导致滑膜炎和关节软骨损伤，滑膜中IL-15的过度表达可能与创伤后骨关节炎有关。
