Siah Ahmed, Morrison Diane B, Fringuelli Elena, Savage Paul, Richmond Zina, Johns Robert, Purcell Maureen K, Johnson Stewart C, Saksida Sonja M
British Columbia Centre for Aquatic Health Sciences, Campbell River, British Columbia, Canada.
Marine Harvest Canada, Campbell River, British Columbia, Canada.
PLoS One. 2015 Nov 4;10(11):e0141475. doi: 10.1371/journal.pone.0141475. eCollection 2015.
Piscine reovirus (PRV) is a double stranded non-enveloped RNA virus detected in farmed and wild salmonids. This study examined the phylogenetic relationships among different PRV sequence types present in samples from salmonids in Western Canada and the US, including Alaska (US), British Columbia (Canada) and Washington State (US). Tissues testing positive for PRV were partially sequenced for segment S1, producing 71 sequences that grouped into 10 unique sequence types. Sequence analysis revealed no identifiable geographical or temporal variation among the sequence types. Identical sequence types were found in fish sampled in 2001, 2005 and 2014. In addition, PRV positive samples from fish derived from Alaska, British Columbia and Washington State share identical sequence types. Comparative analysis of the phylogenetic tree indicated that Canada/US Pacific Northwest sequences formed a subgroup with some Norwegian sequence types (group II), distinct from other Norwegian and Chilean sequences (groups I, III and IV). Representative PRV positive samples from farmed and wild fish in British Columbia and Washington State were subjected to genome sequencing using next generation sequencing methods. Individual analysis of each of the 10 partial segments indicated that the Canadian and US PRV sequence types clustered separately from available whole genome sequences of some Norwegian and Chilean sequences for all segments except the segment S4. In summary, PRV was genetically homogenous over a large geographic distance (Alaska to Washington State), and the sequence types were relatively stable over a 13 year period.
鱼类呼肠孤病毒（PRV）是一种在养殖和野生鲑科鱼类中检测到的双链无包膜RNA病毒。本研究调查了加拿大西部和美国（包括美国阿拉斯加、加拿大不列颠哥伦比亚省和美国华盛顿州）鲑科鱼类样本中不同PRV序列类型之间的系统发育关系。对PRV检测呈阳性的组织进行S1片段的部分测序，产生了71个序列，这些序列分为10种独特的序列类型。序列分析显示，这些序列类型之间没有可识别的地理或时间变异。在2001年、2005年和2014年采集的鱼类样本中发现了相同的序列类型。此外，来自阿拉斯加、不列颠哥伦比亚省和华盛顿州鱼类的PRV阳性样本具有相同的序列类型。系统发育树的比较分析表明，加拿大/美国太平洋西北地区的序列与一些挪威序列类型（第二组）形成一个亚组，与其他挪威和智利序列（第一组、第三组和第四组）不同。使用下一代测序方法对不列颠哥伦比亚省和华盛顿州养殖和野生鱼类的代表性PRV阳性样本进行基因组测序。对10个部分片段中的每一个进行单独分析表明，除S4片段外，加拿大和美国的PRV序列类型在所有片段上均与一些挪威和智利序列的可用全基因组序列分开聚类。总之，PRV在很大的地理距离（从阿拉斯加到华盛顿州）上基因同质，并且序列类型在13年期间相对稳定。
