BD Max StaphSR在来自美国九个普查区域146家机构的当代多样菌株收集中筛查耐甲氧西林金黄色葡萄球菌分离株的性能：mecA缺失突变体的流行情况
Performance of BD Max StaphSR for Screening of Methicillin-Resistant Staphylococcus aureus Isolates among a Contemporary and Diverse Collection from 146 Institutions Located in Nine U.S. Census Regions: Prevalence of mecA Dropout Mutants.
作者信息
Mendes Rodrigo E, Watters Amy A, Rhomberg Paul R, Farrell David J, Jones Ronald N
机构信息
JMI Laboratories, North Liberty, Iowa, USA
JMI Laboratories, North Liberty, Iowa, USA.
出版信息
J Clin Microbiol. 2016 Jan;54(1):204-7. doi: 10.1128/JCM.02047-15. Epub 2015 Nov 4.
Abstract
This study determined the performance of BD Max StaphSR and the rate of methicillin-resistant Staphylococcus aureus (MRSA) with an unrecognized staphylococcal cassette chromosome mec (SCCmec) right-extremity junction (MREJ) region among 907 methicillin-resistant S. aureus (MRSA) and 900 methicillin-susceptible S. aureus (MSSA) isolates. The rate of mecA/mecC dropout mutants was also evaluated. Only three MRSA isolates (99.7% sensitivity; 904/907) were classified as MSSA by the BD Max StaphSR assay, due to negative results for MREJ. Eight MSSA isolates (99.1% sensitivity; 892/900) were assigned as MRSA. However, six of these MSSA isolates had the mecA gene confirmed by PCR and sequencing (99.8% sensitivity; 898/900). Overall, 7.1% (64/900) of MSSA isolates showed results compatible with a mecA dropout genotype.
摘要
本研究测定了BD Max StaphSR的性能，以及907株耐甲氧西林金黄色葡萄球菌（MRSA）和900株甲氧西林敏感金黄色葡萄球菌（MSSA）分离株中未识别的葡萄球菌盒式染色体mec（SCCmec）右末端连接（MREJ）区域的耐甲氧西林金黄色葡萄球菌（MRSA）发生率。还评估了mecA/mecC缺失突变体的发生率。由于MREJ检测结果为阴性，BD Max StaphSR检测仅将3株MRSA分离株（敏感性99.7%；904/907）分类为MSSA。8株MSSA分离株（敏感性99.1%；892/900）被判定为MRSA。然而，其中6株MSSA分离株经PCR和测序证实含有mecA基因（敏感性99.8%；898/900）。总体而言，7.1%（64/900）的MSSA分离株显示出与mecA缺失基因型相符的结果。
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