Okoth Ronald, Basu Amit
Department of Chemistry, Brown University, Box H, 324 Brook Street, Providence, RI, 02912, USA.
Methods Mol Biol. 2016;1367:29-37. doi: 10.1007/978-1-4939-3130-9_3.
We describe a protocol for the preparation of glycopolymers derived from the ring-opening polymerization of a norbornene carboxylic acid derivative. Polymerization is followed by attachment of a linker and subsequent glycoconjugation via a triazole-forming azide-alkyne click reaction. The use of a protected amine-terminating agent allows for the attachment of a probe molecule such as a fluorescein dye. The syntheses of a neutral galactopolymer as well a polyanionic poly-3-O-sulfo-galactopolymer are described.
我们描述了一种由降冰片烯羧酸衍生物的开环聚合制备糖聚合物的方案。聚合反应之后连接一个连接基，随后通过形成三唑的叠氮化物-炔烃点击反应进行糖缀合。使用受保护的胺端基试剂可连接诸如荧光素染料之类的探针分子。本文还描述了中性半乳糖聚合物以及聚阴离子聚-3-O-磺基半乳糖聚合物的合成方法。
